CSF-1, RANKL and OPG regulate osteoclastogenesis during murine tooth eruption

J. Heinrich, S. Bsoul, J. Barnes, K. Woodruff, S. Abboud

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

During tooth eruption, osteoclast-mediated bone resorption predominates in alveolar bone along the occlusal surface rather than in bone basal to the tooth. CSF-1, RANKL and OPG, regulatory molecules essential for osteoclastogenesis, are expressed during eruption. However, it is unclear if these cytokines exhibit an expression pattern that correlates with sites of osteoclastogenesis in vivo. To address this issue, mouse mandibles, isolated from 1 to 14 days postnatal, were analysed for osteoclast activity using tartrate-resistant acid phosphatase (TRAP) staining as well as colony-stimulating factor-1 (CSF-1), receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) mRNA expression using in situ hybridisation. Results showed that CSF-1, RANKL and OPG are expressed in a distinct temporal and spatial manner. In the occlusal region, osteoclast activity was maximal at day 5 and correlated with a relative high expression of CSF-1 and RANKL compared to OPG. In basal bone at this time point, osteoclast activity decreased despite persistent CSF-1 expression and was associated with increased expression of OPG compared to RANKL. By day 8, osteoclastogenesis declined and correlated with upregulation of OPG at the occlusal and basal regions, with this effect continuing throughout eruption. These findings suggest that the spatiotemporal pattern and relative abundance of CSF-1, RANKL and OPG during eruption are key determinants of site-specific osteoclast activity in bone surrounding the tooth. Targeting these cytokines to specific regions in alveolar bone may provide a mechanism for regulating osteoclastogenesis in dental disorders associated with altered tooth eruption.

Original languageEnglish (US)
Pages (from-to)897-908
Number of pages12
JournalArchives of Oral Biology
Volume50
Issue number10
DOIs
StatePublished - Oct 2005

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Colony-Stimulating Factor Receptors
RANK Ligand
Tooth Eruption
Osteoprotegerin
Macrophage Colony-Stimulating Factor
Osteogenesis
Osteoclasts
Bone and Bones
Tooth
Cytokines
Bone Resorption
Mandible
In Situ Hybridization
Up-Regulation
Staining and Labeling
Messenger RNA

Keywords

  • CSF-1
  • Gene expression
  • In situ hybridisation
  • OPG
  • Osteoclasts
  • RANKL
  • Tooth eruption

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

CSF-1, RANKL and OPG regulate osteoclastogenesis during murine tooth eruption. / Heinrich, J.; Bsoul, S.; Barnes, J.; Woodruff, K.; Abboud, S.

In: Archives of Oral Biology, Vol. 50, No. 10, 10.2005, p. 897-908.

Research output: Contribution to journalArticle

Heinrich, J, Bsoul, S, Barnes, J, Woodruff, K & Abboud, S 2005, 'CSF-1, RANKL and OPG regulate osteoclastogenesis during murine tooth eruption', Archives of Oral Biology, vol. 50, no. 10, pp. 897-908. https://doi.org/10.1016/j.archoralbio.2005.02.007
Heinrich, J. ; Bsoul, S. ; Barnes, J. ; Woodruff, K. ; Abboud, S. / CSF-1, RANKL and OPG regulate osteoclastogenesis during murine tooth eruption. In: Archives of Oral Biology. 2005 ; Vol. 50, No. 10. pp. 897-908.
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