Crystallization of a chitosanase from Streptomyces N174

Edward Marcotte, P. John Hart, Isabelle Boucher, Ryszard Brzezinski, Jon D. Robertas

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 Å resolution. The crystals belong to the monoclinic space group P21 with unit cell parameters a = 56.4 Å, b = 59.6 Å, c = 86.1 Å and β = 96.6°. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.

Original languageEnglish (US)
Pages (from-to)995-996
Number of pages2
JournalJournal of Molecular Biology
Volume232
Issue number3
DOIs
StatePublished - Jan 1 1993

Keywords

  • Chitosanase
  • Crystallization
  • Streptomyces
  • X-ray analysis

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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