Crystallization of a chitosanase from Streptomyces N174

Edward Marcotte; P. John Hart; Isabelle Boucher; Ryszard Brzezinski; Jon D. Robertas

doi:10.1006/jmbi.1993.1447

Crystallization of a chitosanase from Streptomyces N174

Edward Marcotte, P. John Hart, Isabelle Boucher, Ryszard Brzezinski, Jon D. Robertas

Biochemistry & Structural Biology

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 Å resolution. The crystals belong to the monoclinic space group P2₁ with unit cell parameters a = 56.4 Å, b = 59.6 Å, c = 86.1 Å and β = 96.6°. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.

Original language	English (US)
Pages (from-to)	995-996
Number of pages	2
Journal	Journal of Molecular Biology
Volume	232
Issue number	3
DOIs	https://doi.org/10.1006/jmbi.1993.1447
State	Published - Jan 1 1993

Keywords

Chitosanase
Crystallization
Streptomyces
X-ray analysis

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1006/jmbi.1993.1447

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title = "Crystallization of a chitosanase from Streptomyces N174",

abstract = "Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 {\AA} resolution. The crystals belong to the monoclinic space group P21 with unit cell parameters a = 56.4 {\AA}, b = 59.6 {\AA}, c = 86.1 {\AA} and β = 96.6°. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.",

keywords = "Chitosanase, Crystallization, Streptomyces, X-ray analysis",

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T1 - Crystallization of a chitosanase from Streptomyces N174

AU - Marcotte, Edward

AU - Hart, P. John

AU - Boucher, Isabelle

AU - Brzezinski, Ryszard

AU - Robertas, Jon D.

PY - 1993/1/1

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N2 - Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 Å resolution. The crystals belong to the monoclinic space group P21 with unit cell parameters a = 56.4 Å, b = 59.6 Å, c = 86.1 Å and β = 96.6°. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.

AB - Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 Å resolution. The crystals belong to the monoclinic space group P21 with unit cell parameters a = 56.4 Å, b = 59.6 Å, c = 86.1 Å and β = 96.6°. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.

KW - Chitosanase

KW - Crystallization

KW - Streptomyces

KW - X-ray analysis

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