Abstract
Chitosanases are produced by many soil fungi and bacteria to degrade chitosan present in fungal cell walls. Here, we report the crystallization of a 29,500 dalton protein with chitosan endo-hydrolase activity isolated from Streptomyces N174. The crystals were grown by vapor diffusion. They are mechanically strong and diffract to at least 1.9 Å resolution. The crystals belong to the monoclinic space group P21 with unit cell parameters a = 56.4 Å, b = 59.6 Å, c = 86.1 Å and β = 96.6°. Cell parameters and crystal density are consistent with two chitosanase molecules per asymmetric unit.
Original language | English (US) |
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Pages (from-to) | 995-996 |
Number of pages | 2 |
Journal | Journal of Molecular Biology |
Volume | 232 |
Issue number | 3 |
DOIs | |
State | Published - Jan 1 1993 |
Keywords
- Chitosanase
- Crystallization
- Streptomyces
- X-ray analysis
ASJC Scopus subject areas
- Structural Biology
- Molecular Biology