Crystal structure of an endochitinase from Hordeum vulgare L. seeds

P. John Hart, Arthur F. Monzingo, Michael P. Ready, Stephen R. Ernst, Jon D. Robertus

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Higher plants contain multiple constitutively expressed proteins for defense against infection by viruses, bacteria, and fungi. One such class of proteins, the chitinases, are effective antifungal agents because they hydrolyze the insoluble β - 1,4-linked polymer of N-acetylglucosamine (chitin), which is the major component of the mycelial cell wall of many fungi. We report here the three-dimensional, 2.8 Å, crystal structure of a 26 kDa endochitinase from barley (Hordeum vulgare L.) seeds. The 243 amino acid residue molecule is rich in α-helices and has three disulfide bonds. A prominent elongated cleft runs the length of the molecule, and is presumably the region responsible for substrate binding and catalysis. Endochitinases from various species of plants show a high degree of similarity in their amino acid sequences. It is therefore likely that the barley endochitinase structure can serve as a model for other plant endochitinases and that catalytic models based on that structure will be applicable to the entire enzyme family.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalJournal of Molecular Biology
Volume229
Issue number1
StatePublished - 1993

Fingerprint

Chitinases
Hordeum
Seeds
Chitin
Acetylglucosamine
Mycoses
Antifungal Agents
Virus Diseases
Catalysis
Disulfides
Cell Wall
Amino Acid Sequence
Polymers
Proteins
Fungi
Bacteria
Amino Acids
Enzymes

Keywords

  • Barley
  • Crystal structure
  • Endochitinase
  • X-ray analysis

ASJC Scopus subject areas

  • Virology

Cite this

Hart, P. J., Monzingo, A. F., Ready, M. P., Ernst, S. R., & Robertus, J. D. (1993). Crystal structure of an endochitinase from Hordeum vulgare L. seeds. Journal of Molecular Biology, 229(1), 189-193.

Crystal structure of an endochitinase from Hordeum vulgare L. seeds. / Hart, P. John; Monzingo, Arthur F.; Ready, Michael P.; Ernst, Stephen R.; Robertus, Jon D.

In: Journal of Molecular Biology, Vol. 229, No. 1, 1993, p. 189-193.

Research output: Contribution to journalArticle

Hart, PJ, Monzingo, AF, Ready, MP, Ernst, SR & Robertus, JD 1993, 'Crystal structure of an endochitinase from Hordeum vulgare L. seeds', Journal of Molecular Biology, vol. 229, no. 1, pp. 189-193.
Hart PJ, Monzingo AF, Ready MP, Ernst SR, Robertus JD. Crystal structure of an endochitinase from Hordeum vulgare L. seeds. Journal of Molecular Biology. 1993;229(1):189-193.
Hart, P. John ; Monzingo, Arthur F. ; Ready, Michael P. ; Ernst, Stephen R. ; Robertus, Jon D. / Crystal structure of an endochitinase from Hordeum vulgare L. seeds. In: Journal of Molecular Biology. 1993 ; Vol. 229, No. 1. pp. 189-193.
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