Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange

Jingfei Xu, Lingyun Zhao, Yuanyuan Xu, Weixing Zhao, Patrick Sung, Hong Wei Wang

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

The central step in eukaryotic homologous recombination (HR) is ATP-dependent DNA-strand exchange mediated by the Rad51 recombinase. In this process, Rad51 assembles on single-stranded DNA (ssDNA) and generates a helical filament that is able to search for and invade homologous double-stranded DNA (dsDNA), thus leading to strand separation and formation of new base pairs between the initiating ssDNA and the complementary strand within the duplex. Here, we used cryo-EM to solve the structures of human RAD51 in complex with DNA molecules, in presynaptic and postsynaptic states, at near-atomic resolution. Our structures reveal both conserved and distinct structural features of the human RAD51-DNA complexes compared with their prokaryotic counterpart. Notably, we also captured the structure of an arrested synaptic complex. Our results provide new insight into the molecular mechanisms of the DNA homology search and strand-exchange processes.

Original languageEnglish (US)
Pages (from-to)40-46
Number of pages7
JournalNature Structural and Molecular Biology
Volume24
Issue number1
DOIs
StatePublished - Jan 5 2017
Externally publishedYes

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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