Cross-species application of cDNA microarrays to profile gene expression using UV-induced melanoma in Monodelphis domestica as the model system

Zhiqiang Wang, Thomas P. Dooley, Ernest V. Curto, Richard L. Davis, John L. Vandeberg

    Research output: Contribution to journalArticle

    23 Scopus citations

    Abstract

    This study established the utility of cross-species application of the cDNA microarray technique for investigating differential gene expression. Using both total RNA and mRNA samples recovered from two opossum cell lines derived from UVB-induced melanoma, we analyzed expression of ca. 4400 genes on the human DermArray DNA microarrays. The signals generated on the DermArrays were clear, strong, and reproducible. A cDNA dot blot consisting of differentially expressed genes representative of different functional clusters was used to validate the DermArray results. We also cloned a Monodelphis gene, keratin 18 (KRT18), and characterized its expression patterns in tumor samples of different progression stages. Up-regulated expression was observed for the KRT18 gene in advanced melanomas, a finding consistent with the DermArray analysis. These results provide evidence that cross-species application of cDNA microarrays is a useful strategy for investigating gene expression patterns in animal models for which species-specific cDNA microarrays are not available.

    Original languageEnglish (US)
    Pages (from-to)588-599
    Number of pages12
    JournalGenomics
    Volume83
    Issue number4
    DOIs
    StatePublished - Apr 1 2004

    Keywords

    • Cross-species
    • DermArray
    • KRT18
    • Monodelphis domestica
    • UV-induced melanoma

    ASJC Scopus subject areas

    • Genetics

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