Covalent linkage of the type- and group-specific antigens to the peptide moiety of the peptidoglycan of serotype III group B Streptococcus

Maria K. Yeung, Stephen J. Mattingly

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6 Scopus citations

Abstract

The attachment sites for the two major cell wall polysaccharides, the type-and group-specific antigens of a serotype III group B streptococcus (GBS) were investigated with [14C]lysine to label the peptide portion of the peptidoglycan and [3H]acetate to label both polysaccharide antigens as well as the glycan backbone of the peptidoglycan. Mutanolysin-treated cell walls were subjected to trypsin digestion, followed by exhaustive beta-elimination with 6 N ammonium hydroxide at 37°C. The resulting products were purified by column chromatography prior to chemical, immunological, and high-voltage electrophoresis analyses. Data from these studies indicated that both cell wall polymers are covalently attached to the peptidoglycan via the peptide unit. Additionally, during synthesis and assembly both antigens attached only to nascent peptidoglycan.

Original languageEnglish (US)
Pages (from-to)205-211
Number of pages7
JournalCurrent Microbiology
Volume14
Issue number4
DOIs
StatePublished - Jul 1986

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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