Correlation of plasma propylthiouracil levels with inhibition of thyroid hormone synthesis in the rat

Gary L. Francis; Owen M. Rennert

doi:10.1016/0026-0495(80)90164-X

Correlation of plasma propylthiouracil levels with inhibition of thyroid hormone synthesis in the rat

Gary L. Francis, Owen M. Rennert

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The inhibition of thyroid hormone synthesis was studied in relation to plasma levels of 6-n-propyl-2-thiouracil (PTU). Na ¹²⁵I (5 μCi) was injected i.p. into adult male Sprague-Dawley rats. After 30 min, graded doses of PTU (0.2 mg/kg, 0.1 mg/kg, and 0.05 mg/kg) were similarly injected. Thyroid hormone synthesis was followed by the accumulation of radioactivity into thyroid glands, which were removed at specified time intervals. PTU levels were measured spectrophotometrically at the time of sacrifice. Additionally, PTU (³⁵S) was used to confirm blood levels of PTU and also to follow intrathyroidal PTU levels. Plasma PTU levels in excess of 0.18 μg/ml completely inhibited thyroid hormone synthesis. Levels between 0.14 and 0.09 μg/ml had a partial effect, and PTU levels less than 0.09 μg/ml had no effect on thyroid hormone synthesis.

Original language	English (US)
Pages (from-to)	410-414
Number of pages	5
Journal	Metabolism
Volume	29
Issue number	5
DOIs	https://doi.org/10.1016/0026-0495(80)90164-X
State	Published - May 1980
Externally published	Yes

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Endocrinology

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title = "Correlation of plasma propylthiouracil levels with inhibition of thyroid hormone synthesis in the rat",

abstract = "The inhibition of thyroid hormone synthesis was studied in relation to plasma levels of 6-n-propyl-2-thiouracil (PTU). Na 125I (5 μCi) was injected i.p. into adult male Sprague-Dawley rats. After 30 min, graded doses of PTU (0.2 mg/kg, 0.1 mg/kg, and 0.05 mg/kg) were similarly injected. Thyroid hormone synthesis was followed by the accumulation of radioactivity into thyroid glands, which were removed at specified time intervals. PTU levels were measured spectrophotometrically at the time of sacrifice. Additionally, PTU (35S) was used to confirm blood levels of PTU and also to follow intrathyroidal PTU levels. Plasma PTU levels in excess of 0.18 μg/ml completely inhibited thyroid hormone synthesis. Levels between 0.14 and 0.09 μg/ml had a partial effect, and PTU levels less than 0.09 μg/ml had no effect on thyroid hormone synthesis.",

author = "Francis, {Gary L.} and Rennert, {Owen M.}",

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AU - Francis, Gary L.

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N2 - The inhibition of thyroid hormone synthesis was studied in relation to plasma levels of 6-n-propyl-2-thiouracil (PTU). Na 125I (5 μCi) was injected i.p. into adult male Sprague-Dawley rats. After 30 min, graded doses of PTU (0.2 mg/kg, 0.1 mg/kg, and 0.05 mg/kg) were similarly injected. Thyroid hormone synthesis was followed by the accumulation of radioactivity into thyroid glands, which were removed at specified time intervals. PTU levels were measured spectrophotometrically at the time of sacrifice. Additionally, PTU (35S) was used to confirm blood levels of PTU and also to follow intrathyroidal PTU levels. Plasma PTU levels in excess of 0.18 μg/ml completely inhibited thyroid hormone synthesis. Levels between 0.14 and 0.09 μg/ml had a partial effect, and PTU levels less than 0.09 μg/ml had no effect on thyroid hormone synthesis.

AB - The inhibition of thyroid hormone synthesis was studied in relation to plasma levels of 6-n-propyl-2-thiouracil (PTU). Na 125I (5 μCi) was injected i.p. into adult male Sprague-Dawley rats. After 30 min, graded doses of PTU (0.2 mg/kg, 0.1 mg/kg, and 0.05 mg/kg) were similarly injected. Thyroid hormone synthesis was followed by the accumulation of radioactivity into thyroid glands, which were removed at specified time intervals. PTU levels were measured spectrophotometrically at the time of sacrifice. Additionally, PTU (35S) was used to confirm blood levels of PTU and also to follow intrathyroidal PTU levels. Plasma PTU levels in excess of 0.18 μg/ml completely inhibited thyroid hormone synthesis. Levels between 0.14 and 0.09 μg/ml had a partial effect, and PTU levels less than 0.09 μg/ml had no effect on thyroid hormone synthesis.

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