Correlation of fragile histidine triad (Fhit) protein structural features with effector interactions and biological functions

Flavia Pichiorri, Hiroshi Okumura, Tatsuya Nakamura, Preston N. Garrison, Pierluigi Gasparini, Sung Suk Suh, Teresa Druck, Kelly A. McCorkell, Larry D. Barnes, Carlo M. Croce, Kay Huebner

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

We have previously shown that Fhit tumor suppressor protein interacts with Hsp60 chaperone machinery and ferredoxin reductase (Fdxr) protein. Fhit-effector interactions are associated with a Fhit-dependent increase in Fdxr stability, followed by generation of reactive oxygen species and apoptosis induction under conditions of oxidative stress. To define Fhit structural features that affect interactions, downstream signaling, and biological outcomes, we used cancer cells expressing Fhit mutants with amino acid substitutions that alter enzymatic activity, enzyme substrate binding, or phosphorylation at tyro-sine 114. Gastric cancer cell clones stably expressing mutants that do not bind substrate or cannot be phosphorylated showed decreased binding to Hsp60 and Fdxr and reduced mitochondrial localization. Expression of Fhit or mutants that bind inter-actor proteins results in oxidative damage and accumulation of cells in G 2/M or sub-G 1 fractions after peroxide treatment; non-interacting mutants are defective in these biological effects. Gastric cancer clones expressing noncomplexing Fhit mutants show reduction of Fhit tumor suppressor activity, confirming that substrate binding, interaction with heat shock proteins, mitochondrial localization, and interaction with Fdxr are important for Fhit tumor suppressor function.

Original languageEnglish (US)
Pages (from-to)1040-1049
Number of pages10
JournalJournal of Biological Chemistry
Volume284
Issue number2
DOIs
StatePublished - Jan 9 2009

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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