Correlation between presence of Trypanosoma cruzi DNA in heart tissue of baboons and cynomolgus monkeys, and lymphocytic myocarditis

James N. Mubiru, Alice Yang, Edward J. Dick, Michael Owston, R. Mark Sharp, Jane F. VandeBerg, Robert E. Shade, John L. VandeBerg

    Research output: Contribution to journalArticlepeer-review

    11 Scopus citations

    Abstract

    Trypanosoma cruzi, the causative agent of Chagas' disease, preferentially infects cardiac and digestive tissues. Baboons living in Texas (Papio hamadryas) and cynomolgus monkeys (Macaca fascicularis) have been reported to be infected naturally with T. cruzi. In this study, we retrospectively reviewed cases of animals that were diagnosed with lymphocytic myocarditis and used a polymerase chain reaction (PCR)-based method (S36/S35 primer set) to amplify T. cruzi DNA from archived frozen and formalin-fixed paraffin-embedded (FFPE) cardiac tissues. We show that the PCR method is applicable in archived frozen and FFPE tissues and the sensitivity is in the femtogram range. A positive correlation between PCR positivity and lymphocytic myocarditis in both baboons and cynomolgus monkeys is shown. We also show epicarditis as a common finding in animals infected with T. cruzi.

    Original languageEnglish (US)
    Pages (from-to)627-633
    Number of pages7
    JournalAmerican Journal of Tropical Medicine and Hygiene
    Volume90
    Issue number4
    DOIs
    StatePublished - Apr 2014

    ASJC Scopus subject areas

    • Parasitology
    • Virology
    • Infectious Diseases

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