Correlated expression of atrial myosin heavy chain and regulatory light chain isoforms with pressure overload hypertrophy in the non-human primate

Richard D. Henkel, Candace M. Kammerer, Laura V. Escobedo, John L. VandeBerg, Richard A. Walsh

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: The aim was to determine the extent to which myosin heavy chain and light chain isoform transitions in atrial myocardium are coordinately regulated under pathological conditions in tissue from normal baboons, hypertensive baboons with myocardial hypertrophy, and baboons in which hypertrophy had regressed. Methods: Quantitative distributions of myosin heavy chain (MHC) and regulatory myosin light chain (MLC2) isoforms in atrial myocardium from 35 adult baboons were determined by electrophoresis under denaturing conditions and laser densitometry. Results: A significant association was observed between the ratios of MHC and MLC2 isoforms in atrial myocardium (r=0.73, p<0.001, n=69). Expressions of a MHC and atrial MLC2 (ALC2) isoforms were correlated in atrial myocardium, as were those of β MHC and ventricular MLC2 (VLC2) isoforms. In a subset of baboons with experimentally induced renal hypertension (n=12) both β MHC and VLC2 isoforms were found at higher levels in left atria than were present in normotensive baboons (p=0.006, n=15). Left atria from hypertensive baboons with regressed LVH contained intermediate levels of both β MHC and VLC2 isoforms. Conclusions: There is tight coupling between the expression of myosin subunit isoforms under pathological conditions from a primate species closely related to humans. The data suggest that the synthesis of these subunits of myosin may be coordinated at the molecular level.

Original languageEnglish (US)
Pages (from-to)416-422
Number of pages7
JournalCardiovascular Research
Volume27
Issue number3
StatePublished - 1993
Externally publishedYes

Keywords

  • Myosin heavy chain
  • Myosin light chain isoforms

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Physiology

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