Correction of chromosomal mutation and random integration in embryonic stem cells with helper-dependent adenoviral vectors

Fumi Ohbayashi, Michael A. Balamotis, Atsuhiro Kishimoto, Emi Aizawa, Arturo Diaz, Paul Hasty, Frank L. Graham, C. Thomas Caskey, Kohnosuke Mitani

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

For gene therapy of inherited diseases, targeted integration/gene repair through homologous recombination (HR) between exogenous and chromosomal DNA would be an ideal strategy to avoid potentially serious problems of random integration such as cellular transformation and gene silencing. Efficient sequence-specific modification of chromosomes by HR would also advance both biological studies and therapeutic applications of a variety of stem cells. Toward these goals, we developed an improved strategy of adenoviral vector (AdV)-mediated HR and examined its ability to correct an insertional mutation in the hypoxanthine phosphoribosyl transferase (Hprt) locus in male mouse ES cells. The efficiency of HR was compared between four types of AdVs that contained various lengths of homologies at the Hprt locus and with various multiplicities of infections. The frequency of HR with helper-dependent AdVs (HD AdVs) with an 18.6-kb homology reached 0.2% per transduced cell at a multiplicity of infection of 10 genomes per cell. Detection of random integration at DNA levels by PCR revealed extremely high efficiency of 5% per cell. We also isolated and characterized chromosomal sites where HD AdVs integrated in a random manner. In contrast to retroviral, lentiviral, and adeno-associated viral vectors, which tend to integrate into genes, the integration sites of AdV was distributed randomly inside and outside genes. These findings suggest that HR mediated by HD AdVs is efficient and relatively safe and might be a new viable option for ex vivo gene therapy as well as a tool for chromosomal manipulation of a variety of stem cells.

Original languageEnglish (US)
Pages (from-to)13628-13633
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume102
Issue number38
DOIs
StatePublished - Sep 20 2005

Keywords

  • Gene therapy
  • Homologous recombination
  • Hypoxanthine phosphoribosyl transferase

ASJC Scopus subject areas

  • General

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