Contribution of FcϵRI-associated vesicles to mast cell-macrophage communication following Francisella tularensis infection

Annette R. Rodriguez, Jieh Juen Yu, Christopher Navara, James P. Chambers, M. Neal Guentzel, Bernard P. Arulanandam

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Understanding innate immune intercellular communication following microbial infection remains a key biological issue. Using live cell imaging, we demonstrate that mast cells actively extend cellular projections to sample the macrophage periphery during Francisella tularensis LVS infection. Mast cell MHCIIhi expression was elevated from less than 1% to 13% during LVS infection. Direct contact during co-culture with macrophages further increased mast cell MHCIIhi expression to approximately 87%. Confocal analyses of the cellular perimeter revealed mast cell caspase-1 was localized in close proximity with FcϵRI in uninfected mast cells, and repositioned to clustered regions upon LVS infection. Importantly, mast cell FcϵRI-encompassed vesicles are transferred to macrophages by trogocytosis, and macrophage caspase-1 expression is further up-regulated upon direct contact with mast cells. Our study reveals direct cellular interactions between innate cells that may impact the function of caspase-1, a known sensor of microbial danger and requirement for innate defense against many pathogenic microbes including F. tularensis.

Original languageEnglish (US)
Pages (from-to)567-574
Number of pages8
JournalInnate Immunity
Volume22
Issue number7
DOIs
StatePublished - Oct 1 2016

Keywords

  • FcϵRI
  • Francisella
  • macrophages
  • mast cells
  • trogocytosis

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Contribution of FcϵRI-associated vesicles to mast cell-macrophage communication following Francisella tularensis infection'. Together they form a unique fingerprint.

  • Cite this