Contribution of actin cytoskeletal alterations to ATP depletion and calcium-induced proximal tubule cell injury

Saul Nurko, Keizo Sogabe, Julie A. Davis, Nancy F. Roeser, Michael Defrain, Alexander Chien, Daniel Hinshaw, Brian Athey, Walter Meixner, Manjeri A. Venkatachalam, Joel M. Weinberg

Research output: Contribution to journalArticle

40 Scopus citations

Abstract

-The actin cytoskeleton of rabbit proximal tubules was assessed by deoxyribonuclease(DNase) binding, sedimentability of detergent-insoluble actin, laser-scanning confocal microscopy, and ultrastructure during exposure to hypoxia, antimycin, or antimycin plus ionomycin. One-third of total actin was DNase reactive in control cells prior to deliberate depolymerization, and a similar proportion was unsedimentable from detergent lysates during 2.5 h at 100,000 g. Tubules injured by hypoxia or antimycin alone, without glycine, showed Ca2+-dependent pathology of the cytoskeleton, consisting of increases in DNase-reactive actin. redistribution of pelletable actin, and loss of microvilli concurrent with lethal membrane damage. In contrast, tubules similarly depleted of ATP and incubated with glycine showed no significant changes of DNase-reactive actin or actin sedimentability for up to 60 min, but, nevertheless, developed substantial loss of basal membrane-associated actin within 15 min and disruption of actin cores and clubbing of microvilli at durations >30 min. These structural changes that occurred in the presence of glycine were not prevented by limiting Ca2+ availability or pH 6.9. Very rapid and extensive cytoskeletal disruption followed antimycin-plusionomycin treatment. In this setting, glycine and pH 6.9 decreased lethal membrane damage but did not ameliorate pathology in the cytoskeleton or microvilli; limiting Ca2+ availability partially protected the cytoskeleton but did not prevent lethal membrane damage. The data suggest that both ATP depletion-dependent but Ca2+ -independent, as well as Ca2+-mediated, processes can disrupt the actin cytoskeleton during acute proximal tubule cell injury; that both types of change occur, despite protection afforded by glycine and reduced pH against lethal membrane damage; and that Ca2+-independent processes primarily account for prelethal actin cytoskeletal alterations during simple ATP depletion of proximal tubule cells.

Original languageEnglish (US)
Pages (from-to)F39-F52
JournalAmerican Journal of Physiology
Volume270
Issue number1 PART 2
DOIs
StatePublished - 1996
Externally publishedYes

Keywords

  • Acidosis
  • Adenosine 5′;-triphosphate
  • Glycine
  • Hypoxia
  • Rabbit

ASJC Scopus subject areas

  • Physiology (medical)

Fingerprint Dive into the research topics of 'Contribution of actin cytoskeletal alterations to ATP depletion and calcium-induced proximal tubule cell injury'. Together they form a unique fingerprint.

  • Cite this

    Nurko, S., Sogabe, K., Davis, J. A., Roeser, N. F., Defrain, M., Chien, A., Hinshaw, D., Athey, B., Meixner, W., Venkatachalam, M. A., & Weinberg, J. M. (1996). Contribution of actin cytoskeletal alterations to ATP depletion and calcium-induced proximal tubule cell injury. American Journal of Physiology, 270(1 PART 2), F39-F52. https://doi.org/10.1152/ajprenal.1996.270.1.f39