Contact‐stimulated proliferation of cultured mouse epidermal cells by 3T3 feeder layers: Inhibition of proliferation by 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA)

Don R. Miller, Kathy M. Hamby, Thomas J. Slaga

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Mouse epidermal cells can be subcultured at 31°C onto an irradiated BALB/c 3T3 clone A31 feeder layer. A31 cells (supposedly derived from embryonic fibroblasts) were found to be specifically required for the optimal production of keratinizing epidermal colonies in secondary culture. This effect was not transmitted through the medium nor by the culture surface, since A31 cells plated on one end of a flask did not stimulate epidermal cell proliferation at the other end, even if the other end had previously held A31 cells. Epidermal cell contact with metabolizing A31 cells was probably necessary for the effect; fixed or freeze‐thawed A31 cells were ineffective. The tumor promoter 12‐O‐tetradecanoylphorbol‐13‐acetate, recently shown to interfere with contact‐mediated transfer of label (metabolic cooperation) between Swiss 3T3 cells and cells of an established epidermal line in vitro, also blocked epidermal colony formation. The A31‐epidermal cell interaction is apparently not a typical mesenchymal‐epithelial interaction, since the basement membrane would prevent this contact in intact skin.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalJournal of Cellular Physiology
Volume112
Issue number1
DOIs
StatePublished - Jul 1982
Externally publishedYes

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

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