Construction of cDNA libraries from limiting amounts of material

John R. McCarrey; Steven A. Williams

doi:10.1016/S0958-1669(05)80067-9

Construction of cDNA libraries from limiting amounts of material

John R. McCarrey, Steven A. Williams

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The use of cDNA libraries has become increasingly widespread as new techniques for library construction and analysis have become available. In recent years, interest in cell-type or stage-specific gene expression has necessitated the construction of cDNA libraries from very small numbers of cells. Recent advances in techniques of RNA isolation, cDNA synthesis, library construction, and the use of the polymerase chain reaction have made this a realistic goal.

Original language	English (US)
Pages (from-to)	34-39
Number of pages	6
Journal	Current Opinion in Biotechnology
Volume	5
Issue number	1
DOIs	https://doi.org/10.1016/S0958-1669(05)80067-9
State	Published - Feb 1994
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering

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10.1016/S0958-1669(05)80067-9

Cite this

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abstract = "The use of cDNA libraries has become increasingly widespread as new techniques for library construction and analysis have become available. In recent years, interest in cell-type or stage-specific gene expression has necessitated the construction of cDNA libraries from very small numbers of cells. Recent advances in techniques of RNA isolation, cDNA synthesis, library construction, and the use of the polymerase chain reaction have made this a realistic goal.",

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Construction of cDNA libraries from limiting amounts of material

Abstract

ASJC Scopus subject areas

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