Construction of cDNA libraries from limiting amounts of material

John R. McCarrey, Steven A. Williams

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

The use of cDNA libraries has become increasingly widespread as new techniques for library construction and analysis have become available. In recent years, interest in cell-type or stage-specific gene expression has necessitated the construction of cDNA libraries from very small numbers of cells. Recent advances in techniques of RNA isolation, cDNA synthesis, library construction, and the use of the polymerase chain reaction have made this a realistic goal.

Original languageEnglish (US)
Pages (from-to)34-39
Number of pages6
JournalCurrent Opinion in Biotechnology
Volume5
Issue number1
DOIs
StatePublished - Feb 1994
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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