Construction of a map of the short arm of human chromosome 6

Robin J Leach, R. DeMars, S. Hasstedt, R. White

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Four DNA sequences that reveal restriction fragment length polymorphisms (RFLPs) on the short arm of human chromosome 6 have been identified. Two of these sequences were isolated from recombinant DNA libraries enriched for DNA from human chromosome 6, one was isolated as a subclone from a human DNA segment having homology to an HLA class I sequence, and one was isolated by virtue of its homology to part of the insulin gene. Genetic linkage was determined among these four polymorphic sequences and several genes known to be on chromosome 6: glyoxylase I (GLO1), HLA-DRα, HLA-DQα, and HLA-B. Additionally, two of the four RFLPs were regionally localized by using 53 deletion cell lines that had been typed for HLA-A, -B, and -DR, and for glyoxylase I. A genetic map of the short arm of chromosome 6 has been constructed on the basis of linkage studies with the eight markers. The map spans a minimum of 60 recombinational units and will be useful in the study of HLA-associated diseases.

Original languageEnglish (US)
Pages (from-to)3909-3913
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume83
Issue number11
StatePublished - 1986
Externally publishedYes

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Chromosomes, Human, Pair 6
Human Chromosomes
HLA-B Antigens
Restriction Fragment Length Polymorphisms
HLA-DQ Antigens
Genetic Linkage
HLA-A Antigens
Recombinant DNA
DNA
HLA-DR Antigens
Gene Library
Genes
Insulin
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Construction of a map of the short arm of human chromosome 6. / Leach, Robin J; DeMars, R.; Hasstedt, S.; White, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, No. 11, 1986, p. 3909-3913.

Research output: Contribution to journalArticle

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