TY - JOUR
T1 - Construction and characterization of a Schistosoma mansoni bacterial artificial chromosome library
AU - Le Paslier, Marie Christine
AU - Pierce, Raymond J.
AU - Merlin, Françoise
AU - Hirai, Hirohisa
AU - Wu, Wenjie
AU - Williams, David L.
AU - Johnston, David
AU - Loverde, Philip T.
AU - Le Paslier, Denis
N1 - Funding Information:
The authors acknowledge Hiroaki Shizuya and Melvin Simon (California Institute of Technology, Pasadena, CA) for providing the pBeloBAC11 vector. We thank Suzanne Van Wingène (Institut Pasteur, Lille, France) and Nathalie Dechamps and Christelle Vaury (Fondation Jean Dausset, CEPH, Paris, France) for their technical assistance. The authors are grateful to Marcel Sala-noubat and Francis Quetier (CNS, Evry, France) for useful advice on developing the BAC end sequencing procedure and to Alain Billault (Fondation Jean Dausset, CEPH, Paris, France), Bruce Birren (Whitehead Institute/MIT, Cambridge, MA), and Pieter de Jong (Roswell Park Cancer Institute, Buffalo, NY) for helpful discussions. We also thank Mark Ross and Danita Pearson (The Sanger Centre, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK) for their help in duplicating and gridding the library. We thank Volvic S.A. for their support in the maintenance of the schistosome life cycle. This work was supported by INSERM, CNRS, by Ohyama Health Foundation (to H.H.), and by UNDP/ World Bank/WHO Special Program for Research and Training in Tropical Diseases Grant 960524.
PY - 2000/4/15
Y1 - 2000/4/15
N2 - A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen. (C) 2000 Academic Press.
AB - A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen. (C) 2000 Academic Press.
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U2 - 10.1006/geno.2000.6147
DO - 10.1006/geno.2000.6147
M3 - Article
C2 - 10783255
AN - SCOPUS:0034655544
VL - 65
SP - 87
EP - 94
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 2
ER -