TY - JOUR
T1 - Construction and characterization of a Schistosoma mansoni bacterial artificial chromosome library
AU - Le Paslier, Marie Christine
AU - Pierce, Raymond J.
AU - Merlin, Françoise
AU - Hirai, Hirohisa
AU - Wu, Wenjie
AU - Williams, David L.
AU - Johnston, David
AU - Loverde, Philip T.
AU - Le Paslier, Denis
PY - 2000/4/15
Y1 - 2000/4/15
N2 - A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen. (C) 2000 Academic Press.
AB - A bacterial artificial chromosome (BAC) library has been established from genomic DNA isolated from the trematode parasite of human, Schistosoma mansoni. This library consists of more than 21,000 recombinant clones carrying inserts in the pBeloBAC11 vector. The mean insert size was 100 kb, representing an approximate 7.95-fold genome coverage. Library screening with eight chromosome-specific or single-copy gene probes yielded between 1 and 9 positive clones, and none of those tested was absent from the library. End sequences were obtained for 93 randomly selected clones, and 37 showed sequence identity to S. mansoni sequences (ESTs, genes, or repetitive sequences). A preliminary analysis by fluorescence in situ hybridization localized 8 clones on schistosome chromosomes 1 (2 clones), 2, 3, 5, Z, and W (3 clones). This library provides a new resource for the physical mapping and sequencing of the genome of this important human pathogen. (C) 2000 Academic Press.
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U2 - 10.1006/geno.2000.6147
DO - 10.1006/geno.2000.6147
M3 - Article
C2 - 10783255
AN - SCOPUS:0034655544
VL - 65
SP - 87
EP - 94
JO - Genomics
JF - Genomics
SN - 0888-7543
IS - 2
ER -