Constitutive calcium-dependent isoform of nitric oxide synthase in the human placental villous vascular tree

L. Myatt, D. E. Brockman, G. Langdon, J. S. Pollock

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

We have characterized the NO synthase enzyme in the villous vasculature of the human placenta as part of our ongoing studies of the regulation of NO synthesis in this circulation. NO synthase activity was determined by conversion of 3H L-arginine to 3H L-citrulline in cellular homogenate, cytosolic and particulate fractions. Optimal NO synthase activity was measured in all fractions in the presence of 1mM NADPH, 10 μM tetrahydrobiopterin, 2,μM FAD, 100 μM fee calcium and 50 U/ml calmodulin. The calmodulin inhibitor calmidazolium (50μM) and FAD inhibitor diphenyliodonium chloride (1 μM) significantly reduced enzyme activity. The EC50 for calcium was 0.1 μM and Km for L-arginine 2.00 ± 0.49 μM with Vmax 55.8 ± 28.3 pmoles/mg protein/min. Enzyme activity was inhibited in both cytosolic and particulate fractions by n(g)-nitro-L-arginine and n(g)-monomethyl-L-arginine in a concentration-dependent manner (10-8-10-(-4M). A calcium-independent NO synthase activity was also determined, but only constituted between 5-6 per cent of total activity. On Western blotting, a single 135 kda species was identified in each fraction with a monoclonal antibody raised against bovine aortic endothelial NO synthase. The NO synthase enzyme of the villous vasculature appears to correspond to the type III calcium-calmodulin dependent endothelial isoform.

Original languageEnglish (US)
Pages (from-to)373-383
Number of pages11
JournalPlacenta
Volume14
Issue number4
StatePublished - 1993
Externally publishedYes

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Nitric Oxide Synthase
Blood Vessels
Protein Isoforms
Calcium
Arginine
Calmodulin
Flavin-Adenine Dinucleotide
Enzymes
calmidazolium
Citrulline
Fees and Charges
NADP
Placenta
Western Blotting
Monoclonal Antibodies
Proteins

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Myatt, L., Brockman, D. E., Langdon, G., & Pollock, J. S. (1993). Constitutive calcium-dependent isoform of nitric oxide synthase in the human placental villous vascular tree. Placenta, 14(4), 373-383.

Constitutive calcium-dependent isoform of nitric oxide synthase in the human placental villous vascular tree. / Myatt, L.; Brockman, D. E.; Langdon, G.; Pollock, J. S.

In: Placenta, Vol. 14, No. 4, 1993, p. 373-383.

Research output: Contribution to journalArticle

Myatt, L, Brockman, DE, Langdon, G & Pollock, JS 1993, 'Constitutive calcium-dependent isoform of nitric oxide synthase in the human placental villous vascular tree', Placenta, vol. 14, no. 4, pp. 373-383.
Myatt, L. ; Brockman, D. E. ; Langdon, G. ; Pollock, J. S. / Constitutive calcium-dependent isoform of nitric oxide synthase in the human placental villous vascular tree. In: Placenta. 1993 ; Vol. 14, No. 4. pp. 373-383.
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