Constancy of masseter muscle structure and function with age in F344 rats

M. Norton, A. Verstegeden, L. C. Maxwell, R. M. McCarter

    Research output: Contribution to journalArticle

    16 Citations (Scopus)

    Abstract

    Senile muscle atrophy is a characteristic feature of advancing age. Despite the growing body of knowledge about weight-bearing muscles in rodents and man, there is relatively little such information on the muscles of mastication. Therefore, the primary goal here was to develop a masseter muscle preparation from male Fischer 344 rats suitable for studying contractile characteristics in vitro. And, secondarily, the goal was to examine this preparation for evidence of age-related changes in muscle composition and function in rats aged 4-24 months. Histochemical analysis of the composition of the four anatomical regions (branches) of the masseter revealed a mixture of rapidly contracting, fatiguable and fatigue-resistant muscle fibres with no significant differences between branches. Fibre type and size were determined with myosin ATPase, NADH-TR and toluidine blue staining of quick- frozen muscle sections. No significant changes in fibre type or fibre area were found with increasing age. One branch of the masseter, the anterior deep masseter, is sufficiently thin (less than 0.8 mm thickness) for adequate diffusion of oxygen and nutrients in studies of isometric contractile properties in vitro. Contraction time, half-relaxation time, dry weight:wet weight ratio and maximum force per unit area were found to be similar in muscles of young and old rats. Our results demonstrate that the anterior deep masseter of the rat is a suitable preparation for investigating masseter function in vitro. The surprising absence of age-related changes in composition and function is consistent with some, but not all, data on ageing rodent limb muscles. The results suggest that masticatory muscle performance is preserved with age in rats.

    Original languageEnglish (US)
    Pages (from-to)139-146
    Number of pages8
    JournalArchives of Oral Biology
    Volume46
    Issue number2
    DOIs
    StatePublished - 2001

    Fingerprint

    Masseter Muscle
    Inbred F344 Rats
    Muscles
    Rodentia
    Masticatory Muscles
    Weights and Measures
    Tolonium Chloride
    Muscle Fatigue
    Muscular Atrophy
    Mastication
    Weight-Bearing
    Frozen Sections
    Myosins
    NAD
    Extremities
    Staining and Labeling
    Oxygen
    Food
    In Vitro Techniques

    Keywords

    • Ageing muscle
    • Fibre types
    • Masseter muscle
    • Rats

    ASJC Scopus subject areas

    • Dentistry(all)

    Cite this

    Constancy of masseter muscle structure and function with age in F344 rats. / Norton, M.; Verstegeden, A.; Maxwell, L. C.; McCarter, R. M.

    In: Archives of Oral Biology, Vol. 46, No. 2, 2001, p. 139-146.

    Research output: Contribution to journalArticle

    Norton, M, Verstegeden, A, Maxwell, LC & McCarter, RM 2001, 'Constancy of masseter muscle structure and function with age in F344 rats', Archives of Oral Biology, vol. 46, no. 2, pp. 139-146. https://doi.org/10.1016/S0003-9969(00)00107-2
    Norton, M. ; Verstegeden, A. ; Maxwell, L. C. ; McCarter, R. M. / Constancy of masseter muscle structure and function with age in F344 rats. In: Archives of Oral Biology. 2001 ; Vol. 46, No. 2. pp. 139-146.
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