Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians

Guillaume Smits; Andrew J. Mungall; Sam Griffiths-Jones; Paul Smith; Delphine Beury; Lucy Matthews; Jane Rogers; Andrew J. Pask; Geoff Shaw; John L. VandeBerg; John R. McCarrey; Marilyn B. Renfree; Wolf Reik; Ian Dunham

doi:10.1038/ng.168

Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians

Guillaume Smits, Andrew J. Mungall, Sam Griffiths-Jones, Paul Smith, Delphine Beury, Lucy Matthews, Jane Rogers, Andrew J. Pask, Geoff Shaw, John L. VandeBerg, John R. McCarrey, Marilyn B. Renfree, Wolf Reik, Ian Dunham

Research output: Contribution to journal › Article

130 Scopus citations

Abstract

Comparisons between eutherians and marsupials suggest limited conservation of the molecular mechanisms that control genomic imprinting in mammals. We have studied the evolution of the imprinted IGF2-H19 locus in therians. Although marsupial orthologs of protein-coding exons were easily identified, the use of evolutionarily conserved regions and low-stringency Bl2seq comparisons was required to delineate a candidate H19 noncoding RNA sequence. The therian H19 orthologs show miR-675 and exon structure conservation, suggesting functional selection on both features. Transcription start site sequences and poly(A) signals are also conserved. As in eutherians, marsupial H19 is maternally expressed and paternal methylation upstream of the gene originates in the male germline, encompasses a CTCF insulator, and spreads somatically into the H19 gene. The conservation in all therians of the mechanism controlling imprinting of the IGF2-H19 locus suggests a sequential model of imprinting evolution.

Original language	English (US)
Pages (from-to)	971-976
Number of pages	6
Journal	Nature Genetics
Volume	40
Issue number	8
DOIs	https://doi.org/10.1038/ng.168
State	Published - Aug 1 2008

ASJC Scopus subject areas

Genetics

Access to Document

10.1038/ng.168

Fingerprint Dive into the research topics of 'Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians'. Together they form a unique fingerprint.

Cite this

Smits, G., Mungall, A. J., Griffiths-Jones, S., Smith, P., Beury, D., Matthews, L., Rogers, J., Pask, A. J., Shaw, G., VandeBerg, J. L., McCarrey, J. R., Renfree, M. B., Reik, W., & Dunham, I. (2008). Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians. Nature Genetics, 40(8), 971-976. https://doi.org/10.1038/ng.168

Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians. / Smits, Guillaume; Mungall, Andrew J.; Griffiths-Jones, Sam; Smith, Paul; Beury, Delphine; Matthews, Lucy; Rogers, Jane; Pask, Andrew J.; Shaw, Geoff; VandeBerg, John L.; McCarrey, John R.; Renfree, Marilyn B.; Reik, Wolf; Dunham, Ian.

In: Nature Genetics, Vol. 40, No. 8, 01.08.2008, p. 971-976.

Research output: Contribution to journal › Article

Smits, Guillaume ; Mungall, Andrew J. ; Griffiths-Jones, Sam ; Smith, Paul ; Beury, Delphine ; Matthews, Lucy ; Rogers, Jane ; Pask, Andrew J. ; Shaw, Geoff ; VandeBerg, John L. ; McCarrey, John R. ; Renfree, Marilyn B. ; Reik, Wolf ; Dunham, Ian. / Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians. In: Nature Genetics. 2008 ; Vol. 40, No. 8. pp. 971-976.

@article{32431909795e4b3c9501ce6e5bad59ed,

title = "Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians",

abstract = "Comparisons between eutherians and marsupials suggest limited conservation of the molecular mechanisms that control genomic imprinting in mammals. We have studied the evolution of the imprinted IGF2-H19 locus in therians. Although marsupial orthologs of protein-coding exons were easily identified, the use of evolutionarily conserved regions and low-stringency Bl2seq comparisons was required to delineate a candidate H19 noncoding RNA sequence. The therian H19 orthologs show miR-675 and exon structure conservation, suggesting functional selection on both features. Transcription start site sequences and poly(A) signals are also conserved. As in eutherians, marsupial H19 is maternally expressed and paternal methylation upstream of the gene originates in the male germline, encompasses a CTCF insulator, and spreads somatically into the H19 gene. The conservation in all therians of the mechanism controlling imprinting of the IGF2-H19 locus suggests a sequential model of imprinting evolution.",

author = "Guillaume Smits and Mungall, {Andrew J.} and Sam Griffiths-Jones and Paul Smith and Delphine Beury and Lucy Matthews and Jane Rogers and Pask, {Andrew J.} and Geoff Shaw and VandeBerg, {John L.} and McCarrey, {John R.} and Renfree, {Marilyn B.} and Wolf Reik and Ian Dunham",

year = "2008",

month = aug,

day = "1",

doi = "10.1038/ng.168",

language = "English (US)",

volume = "40",

pages = "971--976",

journal = "Nature Genetics",

issn = "1061-4036",

publisher = "Nature Publishing Group",

number = "8",

}

TY - JOUR

T1 - Conservation of the H19 noncoding RNA and H19-IGF2 imprinting mechanism in therians

AU - Smits, Guillaume

AU - Mungall, Andrew J.

AU - Griffiths-Jones, Sam

AU - Smith, Paul

AU - Beury, Delphine

AU - Matthews, Lucy

AU - Rogers, Jane

AU - Pask, Andrew J.

AU - Shaw, Geoff

AU - VandeBerg, John L.

AU - McCarrey, John R.

AU - Renfree, Marilyn B.

AU - Reik, Wolf

AU - Dunham, Ian

PY - 2008/8/1

Y1 - 2008/8/1

N2 - Comparisons between eutherians and marsupials suggest limited conservation of the molecular mechanisms that control genomic imprinting in mammals. We have studied the evolution of the imprinted IGF2-H19 locus in therians. Although marsupial orthologs of protein-coding exons were easily identified, the use of evolutionarily conserved regions and low-stringency Bl2seq comparisons was required to delineate a candidate H19 noncoding RNA sequence. The therian H19 orthologs show miR-675 and exon structure conservation, suggesting functional selection on both features. Transcription start site sequences and poly(A) signals are also conserved. As in eutherians, marsupial H19 is maternally expressed and paternal methylation upstream of the gene originates in the male germline, encompasses a CTCF insulator, and spreads somatically into the H19 gene. The conservation in all therians of the mechanism controlling imprinting of the IGF2-H19 locus suggests a sequential model of imprinting evolution.

AB - Comparisons between eutherians and marsupials suggest limited conservation of the molecular mechanisms that control genomic imprinting in mammals. We have studied the evolution of the imprinted IGF2-H19 locus in therians. Although marsupial orthologs of protein-coding exons were easily identified, the use of evolutionarily conserved regions and low-stringency Bl2seq comparisons was required to delineate a candidate H19 noncoding RNA sequence. The therian H19 orthologs show miR-675 and exon structure conservation, suggesting functional selection on both features. Transcription start site sequences and poly(A) signals are also conserved. As in eutherians, marsupial H19 is maternally expressed and paternal methylation upstream of the gene originates in the male germline, encompasses a CTCF insulator, and spreads somatically into the H19 gene. The conservation in all therians of the mechanism controlling imprinting of the IGF2-H19 locus suggests a sequential model of imprinting evolution.

UR - http://www.scopus.com/inward/record.url?scp=48249141249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=48249141249&partnerID=8YFLogxK

U2 - 10.1038/ng.168

DO - 10.1038/ng.168

M3 - Article

C2 - 18587395

AN - SCOPUS:48249141249

VL - 40

SP - 971

EP - 976

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 8

ER -