Connexin expression in epidermal cell lines from SENCAR mouse skin tumors

Irina V. Budunova, Steve Carbajal, Aurora Viaje, Thomas J Slaga

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Alteration of gap-junctional intercellular communication (GJIC) has long been proposed to be involved in carcinogenesis. Previously, we reported that the level of gap junctional intercellular communication in mouse skin carcinoma cell lines is significantly lower than in papilloma cell lines and normal mouse keratinocytes (Klann et al., Cancer Res 49:699-705, 1989). Here, we present data on expression of the gap-junctional protein connexins (Cx) 26, Cx31.1, and Cx43 in a comprehensive panel of keratinocyte cell lines representing different stages of mouse skin carcinogenesis and the effect of different conditions of propagation on Cx phenotype. Northern and western blot analyses and immuno-staining showed that all cell lines studied in vitro expressed Cx43 but most did not express Cx31.1 or Cx26. The abundance of Cx43 expression on plasma membranes correlated well with the level of GJIC. In vivo expression of Cx43 and Cx26 was strongly increased. Whereas none of tumorigenic cell lines expressed Cx26 gap junctions in culture, those growing as tumors in nude mice began to express Cx26 protein. The comparison of Cx expression on the keratinocyte membranes in three different groups of tumors (papillomas and squamous cell and spindle cell carcinomas) clearly revealed that the abundance of Cx43 and Cx26 expression directly correlated with the level of tumor differentiation. All studied tumors were Cx31.1 negative. These results suggest that both Cx expression and gap-junction permeability are gradually reduced during the tumor progression stage of mouse skin carcinogenesis.

Original languageEnglish (US)
Pages (from-to)190-201
Number of pages12
JournalMolecular Carcinogenesis
Volume15
Issue number3
DOIs
StatePublished - Mar 1996
Externally publishedYes

Fingerprint

Inbred SENCAR Mouse
Connexins
Connexin 43
Cell Line
Skin
Keratinocytes
Neoplasms
Carcinogenesis
Gap Junctions
Papilloma
Carcinoma
Nude Mice
Northern Blotting
Permeability
Proteins
Western Blotting
Cell Membrane
Staining and Labeling
Phenotype
Membranes

Keywords

  • Connexins
  • gap junction
  • Keratinocytes
  • Skin tumors

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Connexin expression in epidermal cell lines from SENCAR mouse skin tumors. / Budunova, Irina V.; Carbajal, Steve; Viaje, Aurora; Slaga, Thomas J.

In: Molecular Carcinogenesis, Vol. 15, No. 3, 03.1996, p. 190-201.

Research output: Contribution to journalArticle

Budunova, Irina V. ; Carbajal, Steve ; Viaje, Aurora ; Slaga, Thomas J. / Connexin expression in epidermal cell lines from SENCAR mouse skin tumors. In: Molecular Carcinogenesis. 1996 ; Vol. 15, No. 3. pp. 190-201.
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