Connexin 43 hemichannel opening associated with Prostaglandin E2 release is adaptively regulated by mechanical stimulation

Sirisha Burra, Jean X. Jiang

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Osteocytes present in the bone are known to be the major mechanosensory cells. Their involvement in mechanoregulation of bone remodeling is not yet clear. Osteocytes are connected with each other through gap junctions formed by Connexin 43 (Cx43). Apart from forming gap junctions, Cx43 in osteocytes is also present in the form of hemichannels. Recently, we have developed a unique antibody that specifically blocks hemichannels and does not have any effect on gap junctions. Cx43 hemichannels present in osteocytes of the bone are mechanosensory in nature as they open when subjected to mechanical stimulation in the form of fluid flow shear stress (FFSS). Opening of Cx43 hemichannels results in the release of molecules like Prostaglandin E2 (PGE2) that are involved in bone remodeling. Our recent report shows that the opening of Cx43 hemichannels depends on the magnitude and duration of shear stress. When osteocytes are subjected to FFSS followed by a brief rest and reapplication of FFSS, it led to further increase in opening of Cx43 hemichannels. Application of continuous FFSS for longer periods of time (24 hrs) results in decreased opening of hemichannels. These results show that Cx43 hemichannels are adaptive in response to mechanical stimulation, possibly to regulate the release PGE2 during bone remodeling.

Original languageEnglish (US)
Pages (from-to)239-240
Number of pages2
JournalCommunicative and Integrative Biology
Volume2
Issue number3
DOIs
StatePublished - May 2009

Keywords

  • Adaptive regulation
  • Connexin43
  • Fluid flow shear stress
  • Hemichannels
  • Mechanical stimulation
  • Osteocytes
  • PGE

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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