Conformation of a clathrin triskelion in solution

Matthew L. Ferguson, Kondury Prasad, Dan L. Sackett, Hacène Boukari, Eileen M. Lafer, Ralph Nossal

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19 Scopus citations

Abstract

A principal component in the protein coats of certain post-golgi and endocytic vesicles is clathrin, which appears as a three-legged heteropolymer (known as a triskelion) that assembles into polyhedral cages principally made up of pentagonal and hexagonal faces. In vitro, this assembly depends upon the pH, with cages forming more readily at low pH and less readily at high pH. We have developed procedures, on the basis of static and dynamic light scattering, to determine the radius of gyration, Rg, and hydrodynamic radius, R H, of isolated triskelia, under conditions where cage assembly occurs. Calculations based on rigid molecular bead models of a triskelion show that the measured values can be accounted for by bending the legs and a puckering at the vertex. We also show that the values of Rg and RH measured for clathrin triskelia in solution are qualitatively consistent with the conformation of a triskelion in a "D6 barrel" cage assembly measured by cryoelectron microscopy.

Original languageEnglish (US)
Pages (from-to)5916-5922
Number of pages7
JournalBiochemistry
Volume45
Issue number18
DOIs
StatePublished - May 9 2006

ASJC Scopus subject areas

  • Biochemistry

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    Ferguson, M. L., Prasad, K., Sackett, D. L., Boukari, H., Lafer, E. M., & Nossal, R. (2006). Conformation of a clathrin triskelion in solution. Biochemistry, 45(18), 5916-5922. https://doi.org/10.1021/bi052568w