Confocal scanning laser ophthalmoscopic imaging resolution of secondary retinal effects induced by laser radiation

Harry Zwick, David J. Lund, Bruce E. Stuck, Joseph A. Zuclich, R. Elliott, Steven T. Schuschereba, Donald A.M.D. Gagliano, M. Belkin, Randolph D. Glickman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

We have evaluated secondary laser induced retinal effects in non-human primates with a Rodenstock confocal scanning laser ophthalmoscope. A small eye animal model, the Garter snake, was employed to evaluate confocal numerical aperture effects in imaging laser retinal damage in small eyes vs. large eyes. Results demonstrate that the confocal image resolution in the Rhesus monkey eye is sufficient to differentiate deep retinal scar formation from retinal nerve fiber layer (NFL) damage and to estimate the depth of the NFL damage. The best comparison with histological depth was obtained for the snake retina, yielding a ratio close to 1:1 compared to 2:1 for the Rhesus. Resolution in the Garter snake allows imaging the photoreceptor matrix and therefore, evaluation of the interrelationship between the primary damage site (posterior retina), the photoreceptor matrix, and secondary sites in the anterior retina such as the NFL and the epiretinal vascular system. Alterations in both the retinal NFL and epiretinal blood flow rate were observed within several minutes post Argon laser exposure. Unique aspects of the snake eye such as high tissue transparency and inherently high contrast cellular structures, contribute to the confocal image quality. Such factors may be nearly comparable in primate eyes suggesting that depth of resolution can be improved by smaller confocal apertures and more sensitive signal processing techniques.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsValery V. Tuchin
Pages187-198
Number of pages12
StatePublished - Jan 1 1996
EventCIS Selected Papers: Coherence-Domain Methods in Biomedical Optics -
Duration: Nov 13 1995Nov 13 1995

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume2732
ISSN (Print)0277-786X

Other

OtherCIS Selected Papers: Coherence-Domain Methods in Biomedical Optics
Period11/13/9511/13/95

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Zwick, H., Lund, D. J., Stuck, B. E., Zuclich, J. A., Elliott, R., Schuschereba, S. T., Gagliano, D. A. M. D., Belkin, M., & Glickman, R. D. (1996). Confocal scanning laser ophthalmoscopic imaging resolution of secondary retinal effects induced by laser radiation. In V. V. Tuchin (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (pp. 187-198). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 2732).