Conductivity affects nanosecond electrical pulse induced pressure transient formation

Caleb C. Roth, Ronald A. Barnes, Bennett L. Ibey, Hope T. Beier, Randolph D. Glickman

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Nanoporation occurs in cells exposed to high amplitude short duration (<1μs) electrical pulses. The biophysical mechanism(s) responsible for nanoporation is unknown although several theories exist. Current theories focus exclusively on the electrical field, citing electrostriction, water dipole alignment and/or electrodeformation as the primary mechanisms for pore formation. Our group has shown that mechanical forces of substantial magnitude are also generated during nsEP exposures. We hypothesize that these mechanical forces may contribute to pore formation. In this paper, we report that alteration of the conductivity of the exposure solution also altered the level of mechanical forces generated during a nsEP exposure. By reducing the conductivity of the exposure solutions, we found that we could completely eliminate any pressure transients normally created by nsEP exposure. The data collected for this proceeding does not definitively show that the pressure transients previously identified contribute to nanoporation; however; it indicates that conductivity influences both survival and pressure transient formation.

Original languageEnglish (US)
Title of host publicationOptical Interactions with Tissue and Cells XXVII
PublisherSPIE
Volume9706
ISBN (Electronic)9781628419405
DOIs
Publication statusPublished - 2016
EventOptical Interactions with Tissue and Cells XXVII - San Francisco, United States
Duration: Feb 14 2016Feb 17 2016

Other

OtherOptical Interactions with Tissue and Cells XXVII
CountryUnited States
CitySan Francisco
Period2/14/162/17/16

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Keywords

  • conductivity
  • electrodeformation
  • mechanical stress
  • nanoporation
  • nanosecond electrical pulse

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Roth, C. C., Barnes, R. A., Ibey, B. L., Beier, H. T., & Glickman, R. D. (2016). Conductivity affects nanosecond electrical pulse induced pressure transient formation. In Optical Interactions with Tissue and Cells XXVII (Vol. 9706). [97060V] SPIE. https://doi.org/10.1117/12.2214896