TY - JOUR
T1 - Condensin I and II Complexes License Full Estrogen Receptor α-Dependent Enhancer Activation
AU - Li, Wenbo
AU - Hu, Yiren
AU - Oh, Soohwan
AU - Ma, Qi
AU - Merkurjev, Daria
AU - Song, Xiaoyuan
AU - Zhou, Xiang
AU - Liu, Zhijie
AU - Tanasa, Bogdan
AU - He, Xin
AU - Chen, Aaron Yun
AU - Ohgi, Kenny
AU - Zhang, Jie
AU - Liu, Wen
AU - Rosenfeld, Michael G.
N1 - Publisher Copyright:
© 2015 Elsevier Inc.
PY - 2015/7/16
Y1 - 2015/7/16
N2 - Enhancers instruct spatio-temporally specific gene expression in a manner tightly linked to higher-order chromatin architecture. Critical chromatin architectural regulators condensin I and condensin II play non-redundant roles controlling mitotic chromosomes. But the chromosomal locations of condensins and their functional roles in interphase are poorly understood. Here we report that both condensin complexes exhibit an unexpected, dramatic estrogen-induced recruitment to estrogen receptor α (ER-α)-bound eRNA+ active enhancers in interphase breast cancer cells, exhibiting non-canonical interaction with ER-α via its DNA-binding domain (DBD). Condensins positively regulate ligand-dependent enhancer activation at least in part by recruiting an E3 ubiquitin ligase, HECTD1, to modulate the binding of enhancer-associated coactivators/corepressors, including p300 and RIP140, permitting full eRNA transcription, formation of enhancer:promoter looping, and the resultant coding gene activation. Collectively, our results reveal an important, unanticipated transcriptional role of interphase condensins in modulating estrogen-regulated enhancer activation and coding gene transcriptional program.
AB - Enhancers instruct spatio-temporally specific gene expression in a manner tightly linked to higher-order chromatin architecture. Critical chromatin architectural regulators condensin I and condensin II play non-redundant roles controlling mitotic chromosomes. But the chromosomal locations of condensins and their functional roles in interphase are poorly understood. Here we report that both condensin complexes exhibit an unexpected, dramatic estrogen-induced recruitment to estrogen receptor α (ER-α)-bound eRNA+ active enhancers in interphase breast cancer cells, exhibiting non-canonical interaction with ER-α via its DNA-binding domain (DBD). Condensins positively regulate ligand-dependent enhancer activation at least in part by recruiting an E3 ubiquitin ligase, HECTD1, to modulate the binding of enhancer-associated coactivators/corepressors, including p300 and RIP140, permitting full eRNA transcription, formation of enhancer:promoter looping, and the resultant coding gene activation. Collectively, our results reveal an important, unanticipated transcriptional role of interphase condensins in modulating estrogen-regulated enhancer activation and coding gene transcriptional program.
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U2 - 10.1016/j.molcel.2015.06.002
DO - 10.1016/j.molcel.2015.06.002
M3 - Article
C2 - 26166704
AN - SCOPUS:84937523329
SN - 1097-2765
VL - 59
SP - 188
EP - 202
JO - Molecular Cell
JF - Molecular Cell
IS - 2
ER -