Concurrent down-regulation of Egr-1 and gelsolin in the majority of human breast cancer cells

Jingbo Liu, Ya Guang Liu, Ruochun Huang, Chen Yao, Shiyong Li, Weimin Yang, Dongzi Yang, Ruo Pan Huang

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

A growing body of evidence suggests that early growth response-1 (Egr-1), a transcription factor, may function as a tumor suppressor. The aim of this study was to gain more evidence to support the role of Egr-1 in the suppression of cancer cell growth and to examine the potential correlation between Egr-1 and gelsolin. Materials and Methods: Histochemical staining coupled with breast cancer tissue arrays were used to examine the expression levels of Egr-1 and gebolin. Reporter assays and gel shift were used to study the transcriptional activity of Egr-1 on the regulation of gelsolin. Results: Our data showed that most normal mammary tissues expressed high levels of Egr-1, while the majority of breast cancer tissues expressed very small amounts of Egr-1. The expression pattern of Egr-1 in human breast cancer tissues was highly correlated with gelsolin expression. Induction of Egr-1 by serum stimulation accompanied the increase of gelsolin expression. In cells lacking the induction of Egr-1 in response to serum stimulation, gebolin expression remained unchanged. Furthermore, gebolin promoter activity was profoundly reduced in Egr-1 null mouse embryonic fibroblasts compared to Egr-1 wild-type mouse embryonic fibroblasts. Gel shift experiments indicated that Egr-1 can directly bind to the gelsolin promoter. Conclusion: Our results suggest that Egr-1 may be an important breast cancer marker and that an as yet uncharacterized pathway involved in Egr-1 and gelsolin expression exists which leads to breast cancer cell development.

Original languageEnglish (US)
Pages (from-to)377-386
Number of pages10
JournalCancer Genomics and Proteomics
Volume4
Issue number6
StatePublished - 2007
Externally publishedYes

Keywords

  • Breast cancer
  • Egr-1
  • Gelsolin
  • Gene expression

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cancer Research

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  • Cite this

    Liu, J., Liu, Y. G., Huang, R., Yao, C., Li, S., Yang, W., Yang, D., & Huang, R. P. (2007). Concurrent down-regulation of Egr-1 and gelsolin in the majority of human breast cancer cells. Cancer Genomics and Proteomics, 4(6), 377-386.