Concerted transcriptional regulation by BRCA1 and COBRA1 in breast cancer cells

Sarah E. Aiyar, Hyung Jun Cho, Jae Lee, Rong Li

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Cofactor of BRCA1 (COBRA1) was first identified as a protein that binds to the breast cancer susceptibility gene product BRCA1. COBRA1 modulates estrogen-dependent and independent transcription and suppresses the growth of breast cancer cells. Its expression is significantly reduced in metastatic and recurrent breast cancer, pointing to a tumor suppressor function in breast cancer development. In light of these initial implications of COBRA1 in human breast cancer, the current investigation sought to obtain more direct functional evidence that links COBRA1 with BRCA1 in transcriptional regulation in breast cancer cells. Small hairpin RNA (shRNA)-mediated gene knockdown and gene expression microarray were used to study the impact of COBRA1 and BRCA1 on global transcription in the same breast cancer cell background. The gene expression profiling study in tissue culture cells uncovers a significant overlap of COBRA1- and BRCA1-regulated genes, many of which have been previously implicated in breast cancer progression. The data shown herein support the notion that COBRA1 and BRCA1 may engage in common gene regulatory pathways to suppress breast cancer progression.

Original languageEnglish (US)
Pages (from-to)486-492
Number of pages7
JournalInternational Journal of Biological Sciences
Volume3
Issue number7
DOIs
StatePublished - Nov 26 2007

Keywords

  • BRCA1
  • Breast cancer
  • COBRA1
  • Transcription regulation
  • shRNA knockdown

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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