Comprehensive and quantitative analysis of polyphosphoinositide species by shotgun lipidomics revealed their alterations in db/db mouse brain

Chunyan Wang, Juan Pablo Palavicini, Miao Wang, Linyuan Chen, Kui Yang, Peter A. Crawford, Xianlin Han

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Polyphosphoinositides (PPI) play crucial roles in cellular signaling and functions. However, comprehensively determining the changed levels of these species during different cellular processes has faced difficulties. Herein, we applied a novel methylation pattern recognition and simulation approach, and we exploited newly derived fragmentation patterns of methylated PPI species for comprehensive analysis of PPI species including phosphate position(s) and fatty acyl chains capable of circumpassing previous limitations. The developed method was applied for quantitative analysis of PPI species present in diabetic mouse cortex and liver, and it allowed us to unravel the marked reduction of PPI levels in brain cortices of db/db mice for the first time. Taken together, we developed a powerful and high-throughput method for comprehensive analysis of PPI species, which should greatly contribute to the elucidation of PPI biology under different disease states.

Original languageEnglish (US)
Pages (from-to)12137-12144
Number of pages8
JournalAnalytical Chemistry
Volume88
Issue number24
DOIs
StatePublished - Jan 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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