Abstract
Protein SRP19 is an important component of the signal recognition particle (SRP) as it promotes assembly of protein SRP54 with SRP RNA and recognizes a tetranucleotide loop. Structural features and RNA binding activities of SRP19 of the hyperthermophilic archaeon Archaeoglobus fulgidus were investigated. An updated alignment of SRP19 sequences predicted three conserved regions and two α-helices. With Af-SRP RNA the Af-SRP54 protein assembled into an A. fulgidus SRP which remained intact for many hours. Stable complexes were formed between Af-SRP19 and truncated SRP RNAs, including a 36-residue fragment representing helix 6 of A. fulgidus SRP RNA.
Original language | English (US) |
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Pages (from-to) | 105-110 |
Number of pages | 6 |
Journal | FEMS Microbiology Letters |
Volume | 198 |
Issue number | 2 |
DOIs | |
State | Published - May 1 2001 |
Keywords
- Archaeon
- Protein targeting
- Protein-RNA interaction
- Signal recognition particle
ASJC Scopus subject areas
- Microbiology
- Molecular Biology
- Genetics