Complexes with truncated RNAs from the large domain of Archaeoglobus fulgidus signal recognition particle

Shakhawat Hossain Bhuiyan, Olga N. Pakhomova, Andrew P. Hinck, Christian Zwieb

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Protein SRP19 is an important component of the signal recognition particle (SRP) as it promotes assembly of protein SRP54 with SRP RNA and recognizes a tetranucleotide loop. Structural features and RNA binding activities of SRP19 of the hyperthermophilic archaeon Archaeoglobus fulgidus were investigated. An updated alignment of SRP19 sequences predicted three conserved regions and two α-helices. With Af-SRP RNA the Af-SRP54 protein assembled into an A. fulgidus SRP which remained intact for many hours. Stable complexes were formed between Af-SRP19 and truncated SRP RNAs, including a 36-residue fragment representing helix 6 of A. fulgidus SRP RNA.

Original languageEnglish (US)
Pages (from-to)105-110
Number of pages6
JournalFEMS Microbiology Letters
Volume198
Issue number2
DOIs
StatePublished - May 1 2001

Keywords

  • Archaeon
  • Protein targeting
  • Protein-RNA interaction
  • Signal recognition particle

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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