Complexes with truncated RNAs from the large domain of Archaeoglobus fulgidus signal recognition particle

Shakhawat Hossain Bhuiyan; Olga N. Pakhomova; Andrew P. Hinck; Christian Zwieb

doi:10.1016/S0378-1097(01)00141-0

Complexes with truncated RNAs from the large domain of Archaeoglobus fulgidus signal recognition particle

Shakhawat Hossain Bhuiyan, Olga N. Pakhomova, Andrew P. Hinck, Christian Zwieb

Biochemistry & Structural Biology

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

Protein SRP19 is an important component of the signal recognition particle (SRP) as it promotes assembly of protein SRP54 with SRP RNA and recognizes a tetranucleotide loop. Structural features and RNA binding activities of SRP19 of the hyperthermophilic archaeon Archaeoglobus fulgidus were investigated. An updated alignment of SRP19 sequences predicted three conserved regions and two α-helices. With Af-SRP RNA the Af-SRP54 protein assembled into an A. fulgidus SRP which remained intact for many hours. Stable complexes were formed between Af-SRP19 and truncated SRP RNAs, including a 36-residue fragment representing helix 6 of A. fulgidus SRP RNA.

Original language	English (US)
Pages (from-to)	105-110
Number of pages	6
Journal	FEMS Microbiology Letters
Volume	198
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1097(01)00141-0
State	Published - May 1 2001

Keywords

Archaeon
Protein targeting
Protein-RNA interaction
Signal recognition particle

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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Bhuiyan, S. H., Pakhomova, O. N., Hinck, A. P., & Zwieb, C. (2001). Complexes with truncated RNAs from the large domain of Archaeoglobus fulgidus signal recognition particle. FEMS Microbiology Letters, 198(2), 105-110. https://doi.org/10.1016/S0378-1097(01)00141-0

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abstract = "Protein SRP19 is an important component of the signal recognition particle (SRP) as it promotes assembly of protein SRP54 with SRP RNA and recognizes a tetranucleotide loop. Structural features and RNA binding activities of SRP19 of the hyperthermophilic archaeon Archaeoglobus fulgidus were investigated. An updated alignment of SRP19 sequences predicted three conserved regions and two α-helices. With Af-SRP RNA the Af-SRP54 protein assembled into an A. fulgidus SRP which remained intact for many hours. Stable complexes were formed between Af-SRP19 and truncated SRP RNAs, including a 36-residue fragment representing helix 6 of A. fulgidus SRP RNA.",

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AU - Bhuiyan, Shakhawat Hossain

AU - Pakhomova, Olga N.

AU - Hinck, Andrew P.

AU - Zwieb, Christian

PY - 2001/5/1

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KW - Archaeon

KW - Protein targeting

KW - Protein-RNA interaction

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