Completion of the La crosse virus genome sequence and geneticcomparisons of the L proteins of the Bunyaviridae

Anjeanette Roberts, Colette Rossier, Dan Kolakofsky, Neal Nathanson, Francisco Gonzalez-Scarano

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

La Crosse virus is a member of the Bunyavirus genus in the family Bunyaviridae, viruses with trisegmented RNA genomes of mostly negative polarity composed of large (L), medium (M), and small (S) segments. The sequences of the La Crosse/ original M and S RNA segments have been previously characterized. Using reverse transcriptase in conjunction with PCR amplification, we have obtained the nucleotide sequence of the L RNA segment, which encodes the viral polymerase in a single large open reading frame. Comparison of the amino acid sequence of the LAC L protein with the sequence of other polymerases from members of the Bunyaviridae, demonstrated the presence of several conserved motifs, some of which are characteristic of many polymerase proteins. A genetic tree comparing the available polymerase proteins of the Bunyaviridae provides insights into the phylogenetic relationships within this large family. Members of the genus Bunyavirus, which are mosquito-borne and infect mammals, have a closer relationship to the plant viruses represented by tomato spotted wilt virus (Tospovirus genus) than to viruses of other genera in the family Bunyaviridae.

Original languageEnglish (US)
Pages (from-to)742-745
Number of pages4
JournalVirology
Volume206
Issue number1
DOIs
StatePublished - Jan 10 1995
Externally publishedYes

Fingerprint

La Crosse virus
Bunyaviridae
Tospovirus
Orthobunyavirus
Genome
RNA
Proteins
Viruses
Plant Viruses
RNA-Directed DNA Polymerase
Culicidae
Open Reading Frames
Mammals
Amino Acid Sequence
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Roberts, A., Rossier, C., Kolakofsky, D., Nathanson, N., & Gonzalez-Scarano, F. (1995). Completion of the La crosse virus genome sequence and geneticcomparisons of the L proteins of the Bunyaviridae. Virology, 206(1), 742-745. https://doi.org/10.1016/S0042-6822(95)80001-8

Completion of the La crosse virus genome sequence and geneticcomparisons of the L proteins of the Bunyaviridae. / Roberts, Anjeanette; Rossier, Colette; Kolakofsky, Dan; Nathanson, Neal; Gonzalez-Scarano, Francisco.

In: Virology, Vol. 206, No. 1, 10.01.1995, p. 742-745.

Research output: Contribution to journalArticle

Roberts, A, Rossier, C, Kolakofsky, D, Nathanson, N & Gonzalez-Scarano, F 1995, 'Completion of the La crosse virus genome sequence and geneticcomparisons of the L proteins of the Bunyaviridae', Virology, vol. 206, no. 1, pp. 742-745. https://doi.org/10.1016/S0042-6822(95)80001-8
Roberts, Anjeanette ; Rossier, Colette ; Kolakofsky, Dan ; Nathanson, Neal ; Gonzalez-Scarano, Francisco. / Completion of the La crosse virus genome sequence and geneticcomparisons of the L proteins of the Bunyaviridae. In: Virology. 1995 ; Vol. 206, No. 1. pp. 742-745.
@article{489596a169bc49638dcbd1b988010462,
title = "Completion of the La crosse virus genome sequence and geneticcomparisons of the L proteins of the Bunyaviridae",
abstract = "La Crosse virus is a member of the Bunyavirus genus in the family Bunyaviridae, viruses with trisegmented RNA genomes of mostly negative polarity composed of large (L), medium (M), and small (S) segments. The sequences of the La Crosse/ original M and S RNA segments have been previously characterized. Using reverse transcriptase in conjunction with PCR amplification, we have obtained the nucleotide sequence of the L RNA segment, which encodes the viral polymerase in a single large open reading frame. Comparison of the amino acid sequence of the LAC L protein with the sequence of other polymerases from members of the Bunyaviridae, demonstrated the presence of several conserved motifs, some of which are characteristic of many polymerase proteins. A genetic tree comparing the available polymerase proteins of the Bunyaviridae provides insights into the phylogenetic relationships within this large family. Members of the genus Bunyavirus, which are mosquito-borne and infect mammals, have a closer relationship to the plant viruses represented by tomato spotted wilt virus (Tospovirus genus) than to viruses of other genera in the family Bunyaviridae.",
author = "Anjeanette Roberts and Colette Rossier and Dan Kolakofsky and Neal Nathanson and Francisco Gonzalez-Scarano",
year = "1995",
month = "1",
day = "10",
doi = "10.1016/S0042-6822(95)80001-8",
language = "English (US)",
volume = "206",
pages = "742--745",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Completion of the La crosse virus genome sequence and geneticcomparisons of the L proteins of the Bunyaviridae

AU - Roberts, Anjeanette

AU - Rossier, Colette

AU - Kolakofsky, Dan

AU - Nathanson, Neal

AU - Gonzalez-Scarano, Francisco

PY - 1995/1/10

Y1 - 1995/1/10

N2 - La Crosse virus is a member of the Bunyavirus genus in the family Bunyaviridae, viruses with trisegmented RNA genomes of mostly negative polarity composed of large (L), medium (M), and small (S) segments. The sequences of the La Crosse/ original M and S RNA segments have been previously characterized. Using reverse transcriptase in conjunction with PCR amplification, we have obtained the nucleotide sequence of the L RNA segment, which encodes the viral polymerase in a single large open reading frame. Comparison of the amino acid sequence of the LAC L protein with the sequence of other polymerases from members of the Bunyaviridae, demonstrated the presence of several conserved motifs, some of which are characteristic of many polymerase proteins. A genetic tree comparing the available polymerase proteins of the Bunyaviridae provides insights into the phylogenetic relationships within this large family. Members of the genus Bunyavirus, which are mosquito-borne and infect mammals, have a closer relationship to the plant viruses represented by tomato spotted wilt virus (Tospovirus genus) than to viruses of other genera in the family Bunyaviridae.

AB - La Crosse virus is a member of the Bunyavirus genus in the family Bunyaviridae, viruses with trisegmented RNA genomes of mostly negative polarity composed of large (L), medium (M), and small (S) segments. The sequences of the La Crosse/ original M and S RNA segments have been previously characterized. Using reverse transcriptase in conjunction with PCR amplification, we have obtained the nucleotide sequence of the L RNA segment, which encodes the viral polymerase in a single large open reading frame. Comparison of the amino acid sequence of the LAC L protein with the sequence of other polymerases from members of the Bunyaviridae, demonstrated the presence of several conserved motifs, some of which are characteristic of many polymerase proteins. A genetic tree comparing the available polymerase proteins of the Bunyaviridae provides insights into the phylogenetic relationships within this large family. Members of the genus Bunyavirus, which are mosquito-borne and infect mammals, have a closer relationship to the plant viruses represented by tomato spotted wilt virus (Tospovirus genus) than to viruses of other genera in the family Bunyaviridae.

UR - http://www.scopus.com/inward/record.url?scp=0028896154&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028896154&partnerID=8YFLogxK

U2 - 10.1016/S0042-6822(95)80001-8

DO - 10.1016/S0042-6822(95)80001-8

M3 - Article

C2 - 7831835

AN - SCOPUS:0028896154

VL - 206

SP - 742

EP - 745

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -