Complete nucleotide sequence of an Amerindian human T-cell lymphotropic virus type II (HTLV-II) isolate: Identification of a variant HTLV-II subtype b from a Guaymi Indian

Diane Pardi, William M. Switzer, Kenneth G. Hadlock, Jonathan E. Kaplan, Renu B. Lal, Thomas M. Folks

Research output: Contribution to journalArticle

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Abstract

The complete nucleotide sequence of a human T-cell lymphotropic virus type II (HTLV-II) isolate from a Panamanian Guaymi Indian was determined and analyzed. When this new viral isolate (HTLV-IIG12) was compared with prototypic HTLV-HMoT, the overall nucleotide sequence similarity was 95.4%, while the predicted amino acid sequence similarity was 97.5%. Although the overall percentage of nucleotide and amino acid identity with prototypic HTLV-IIMoT (subtype a) was high, HTLV-IIG12 displayed several distinctive features that defined it as an HTLV-II subtype b. However, there were several characteristics unique to this isolate, which included a cluster of nucleotide substitutions in the pre-gag region and changes in restriction enzyme sites within the pre-gag region and the gag, pol, env, and pX genes. In addition, two nucleotide changes in the C terminus of the Tax protein coding sequence inserted an Arg residue for a stop codon and appeared to result in a larger tax gene product in HTLV-IIG12. Although the HTLV-IIG12 isolate appears to be a variant of the prototypic HTLV-IIb, this information represents the first complete nucleotide sequence of any HTLV-II subtype b. These data will allow further studies on the evolutionary relationships between the HTLV-II subtypes and between HTLV-I and HTLV-II.

Original languageEnglish (US)
Pages (from-to)4659-4664
Number of pages6
JournalJournal of Virology
Volume67
Issue number8
StatePublished - Aug 1993
Externally publishedYes

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Human T-lymphotropic virus 2
American Indians
T-lymphocytes
T-Lymphocytes
nucleotide sequences
viruses
tax Gene Products
Nucleotides
nucleotides
taxes
pX Genes
pol Genes
gag Genes
env Genes
Human T-lymphotropic virus 1
Terminator Codon
stop codon
Amino Acid Sequence
genes
amino acid sequences

ASJC Scopus subject areas

  • Immunology

Cite this

Complete nucleotide sequence of an Amerindian human T-cell lymphotropic virus type II (HTLV-II) isolate : Identification of a variant HTLV-II subtype b from a Guaymi Indian. / Pardi, Diane; Switzer, William M.; Hadlock, Kenneth G.; Kaplan, Jonathan E.; Lal, Renu B.; Folks, Thomas M.

In: Journal of Virology, Vol. 67, No. 8, 08.1993, p. 4659-4664.

Research output: Contribution to journalArticle

Pardi, Diane ; Switzer, William M. ; Hadlock, Kenneth G. ; Kaplan, Jonathan E. ; Lal, Renu B. ; Folks, Thomas M. / Complete nucleotide sequence of an Amerindian human T-cell lymphotropic virus type II (HTLV-II) isolate : Identification of a variant HTLV-II subtype b from a Guaymi Indian. In: Journal of Virology. 1993 ; Vol. 67, No. 8. pp. 4659-4664.
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