Competitive stem cell recruitment by multiple cytotactic cues

Avital Mendelson, Yuk Kee Cheung, Kamila Paluch, Mo Chen, Kimi Kong, Jiali Tan, Ziming Dong, Samuel K. Sia, Jeremy J. Mao

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A multitude of cytotactic cues direct cell migration in development, cancer metastasis and wound healing. However, our understanding of cell motility remains fragmented partially because current migration devices only allow the study of independent factors. We developed a cell motility assay that allows competitive recruitment of a given cell population simultaneously by gradients of multiple cytotactic cues, observable under real-time imaging. Well-defined uniform gradients of cytotactic cues can be independently generated and sustained in each channel. As a case study, bone marrow mesenchymal stem/stromal cells (MSCs) were exposed to 15 cytokines that are commonly present in arthritis. Cytokines that induced robust recruitment of MSCs in multiple groups were selected to 'compete' in a final round to yield the most chemotactic factor(s) based on cell migration numbers, distances, migration indices and motility over time. The potency of a given cytokine in competition frequently differed from its individual action, substantiating the need to test multiple cytokines concurrently due to synergistic or antagonistic effects. This new device has the rare capacity to screen molecules that induce cell migration in cancer therapy, drug development and tissue regeneration.

Original languageEnglish (US)
Pages (from-to)1156-1164
Number of pages9
JournalLab on a Chip
Volume13
Issue number6
DOIs
StatePublished - Mar 21 2013
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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