Comparison of the protective effect of melatonin with other antioxidants in the hamster kidney model of estradiol-induced DNA damage

Małgorzata Karbownik, Russel J. Reiter, Javier Cabrera, Joaquin J. Garcia

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

17β-Estradiol (E2) is a known carcinogen. Estrogen induction of tumors in hamster kidney is a model of estrogen-related carcinogenesis. Melatonin is a well-known antioxidant, free radical scavenger and oncostatic agent. Changes in the levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo), an index of DNA damage, were measured in kidneys, liver and testes from hamsters treated with E2 (75 mg/kg b.w.) and collected 5h later. Potential protective effects of melatonin, N-acetylserotonin (NAS), indole-3-propionic acid (IPA) and ascorbic acid (AA) against E2-induced DNA damage were tested. The antioxidants were applied in equimolar doses of 64.5 μmol/kg b.w., 2 and 0.5 h before and 2 and 4 h after E2 treatment. E2 treatment caused a significant increase in 8-oxodGuo levels in kidneys, but did not influence significantly the oxidation of guanine bases in liver and testes. Melatonin, IPA and AA, but not NAS, completely prevented E2-induced DNA damage in hamster kidneys. It is concluded that melatonin, IPA and AA may be effective in protecting against E2-related DNA damage and, consequently, carcinogenesis.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume474
Issue number1-2
DOIs
StatePublished - Mar 1 2001

Keywords

  • 17β-Estradiol
  • Antioxidants
  • DNA damage
  • Hamster kidney
  • Melatonin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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