Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods

Daniel S.H. Liu, Glenn Hiltibran, Thomas Castle, Arlan Richardson

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.

Original languageEnglish (US)
Pages (from-to)644-648
Number of pages5
JournalAnalytical Biochemistry
Volume88
Issue number2
DOIs
StatePublished - Aug 1 1978

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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