Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods

Daniel S H Liu, Glenn Hiltibran, Thomas Castle, Arlan Richardson

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.

Original languageEnglish (US)
Pages (from-to)644-648
Number of pages5
JournalAnalytical Biochemistry
Volume88
Issue number2
DOIs
StatePublished - Aug 1 1978
Externally publishedYes

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Diethyl Pyrocarbonate
Chloroform
Phenol
Sodium Dodecyl Sulfate
Liver
Rats
RNA
Poly A
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods. / Liu, Daniel S H; Hiltibran, Glenn; Castle, Thomas; Richardson, Arlan.

In: Analytical Biochemistry, Vol. 88, No. 2, 01.08.1978, p. 644-648.

Research output: Contribution to journalArticle

Liu, Daniel S H ; Hiltibran, Glenn ; Castle, Thomas ; Richardson, Arlan. / Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods. In: Analytical Biochemistry. 1978 ; Vol. 88, No. 2. pp. 644-648.
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