Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods

Daniel S.H. Liu; Glenn Hiltibran; Thomas Castle; Arlan Richardson

doi:10.1016/0003-2697(78)90468-2

Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods

Daniel S.H. Liu, Glenn Hiltibran, Thomas Castle, Arlan Richardson

Barshop Institute

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.

Original language	English (US)
Pages (from-to)	644-648
Number of pages	5
Journal	Analytical Biochemistry
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(78)90468-2
State	Published - Aug 1 1978

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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abstract = "A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.",

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AU - Castle, Thomas

AU - Richardson, Arlan

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N2 - A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.

AB - A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.

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Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods

Abstract

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