Abstract
A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 644-648 |
| Number of pages | 5 |
| Journal | Analytical Biochemistry |
| Volume | 88 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1 1978 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods. / Liu, Daniel S H; Hiltibran, Glenn; Castle, Thomas; Richardson, Arlan.
In: Analytical Biochemistry, Vol. 88, No. 2, 01.08.1978, p. 644-648.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparison of the extraction of RNA from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate and phenol-chloroform methods
AU - Liu, Daniel S H
AU - Hiltibran, Glenn
AU - Castle, Thomas
AU - Richardson, Arlan
PY - 1978/8/1
Y1 - 1978/8/1
N2 - A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.
AB - A higher yield of RNA extracted from rat liver by the diethyl pyrocarbonate-sodium dodecyl sulfate method compared to the phenol-chloroform method was consistently obtained. There was no qualitative difference observed in the types of RNA isolated by the two methods. The diethyl pyrocarbonate-sodium dodecyl sulfate technique was shown to be an efficient method for the extraction of poly(A)-containing RNA from rat liver.
UR - http://www.scopus.com/inward/record.url?scp=0018127594&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018127594&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(78)90468-2
DO - 10.1016/0003-2697(78)90468-2
M3 - Article
C2 - 697033
AN - SCOPUS:0018127594
VL - 88
SP - 644
EP - 648
JO - Analytical Biochemistry
JF - Analytical Biochemistry
SN - 0003-2697
IS - 2
ER -