Comparison of quantitative microbiology and histopathology in divided burn-wound biopsy specimens

A. T. McManus, S. H. Kim, W. F. McManus, A. D. Mason, Basil A Pruitt

Research output: Contribution to journalArticle

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Abstract

We examined the association between quantitative microbiologic results and histopathologic findings in divided biopsy specimens from 200 burned patients. Microbiologic counts were determined as log10 colony-forming units per gram of disrupted tissue. Histopathologic results were scored on a scale of 1 to 6, values of 4 or greater indicating microbial invasion of viable tissue. Agreement of 96.1% was found between negative cultures, arbitrarily identified as those with fewer than 5 logs/g, and histologic absence of invasive infection. In sharp contrast, however, histologic invasion occurred in only 36% of specimens with positive cultures. Though low tissue counts are essentially synonymous with negative histologic findings, quantitative microbiology is not a diagnostic substitute for histologic examination, since high tissue counts quite commonly do not indicate invasion. The principal value of quantitative burn-wound biopsies is the demonstration of predominant burn-wound flora.

Original languageEnglish (US)
Pages (from-to)74-76
Number of pages3
JournalArchives of Surgery
Volume122
Issue number1
StatePublished - 1987
Externally publishedYes

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Microbiology
Burns
Biopsy
Wounds and Injuries
Stem Cells
Infection

ASJC Scopus subject areas

  • Surgery

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McManus, A. T., Kim, S. H., McManus, W. F., Mason, A. D., & Pruitt, B. A. (1987). Comparison of quantitative microbiology and histopathology in divided burn-wound biopsy specimens. Archives of Surgery, 122(1), 74-76.

Comparison of quantitative microbiology and histopathology in divided burn-wound biopsy specimens. / McManus, A. T.; Kim, S. H.; McManus, W. F.; Mason, A. D.; Pruitt, Basil A.

In: Archives of Surgery, Vol. 122, No. 1, 1987, p. 74-76.

Research output: Contribution to journalArticle

McManus, AT, Kim, SH, McManus, WF, Mason, AD & Pruitt, BA 1987, 'Comparison of quantitative microbiology and histopathology in divided burn-wound biopsy specimens', Archives of Surgery, vol. 122, no. 1, pp. 74-76.
McManus, A. T. ; Kim, S. H. ; McManus, W. F. ; Mason, A. D. ; Pruitt, Basil A. / Comparison of quantitative microbiology and histopathology in divided burn-wound biopsy specimens. In: Archives of Surgery. 1987 ; Vol. 122, No. 1. pp. 74-76.
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