TY - JOUR
T1 - Comparison of Preterm and Term Wharton's Jelly-Derived Mesenchymal Stem Cell Properties in Different Oxygen Tensions
AU - Balgi-Agarwal, Saloni
AU - Winter, Caitlyn
AU - Corral, Alexis
AU - Mustafa, Shamimunisa B.
AU - Hornsby, Peter
AU - Moreira, Alvaro
N1 - Funding Information:
The project described was supported by the National Center for Advancing Translational Sciences, National Institutes of Health (grant KL2 TR001118). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. This study was also supported by a UT Health–San Antonio School of Medicine Clinical Investigator Kickstart Pilot Grant.
Funding Information:
Data were generated in the Flow Cytometry Shared Resource Facility, which is supported by UT Health-Science Center at San Antonio (UTHSCSA), NIH-NCI P30 CA054174-20 (Cancer Therapy and Research Center at UTHSCSA) and UL1 TR001120 (Clinical and Translational Science Award).
Publisher Copyright:
© 2018 © 2018 S. Karger AG, Basel. Copyright: All rights reserved.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Mesenchymal stem cells (MSCs) have shown promise as therapeutic agents in treating morbidities associated with premature birth. MSCs derived from the human umbilical cord are easy to isolate and have low immunogenicity and a robust ability to secrete paracrine factors. To date, there are no studies evaluating preterm versus term umbilical cord tissue-derived MSCs. Therefore, our aim was twofold: (1) to compare stem cell properties in preterm versus term MSCs and (2) to examine the impact of oxygen tension on stem cell behavior. Umbilical cord tissue was obtained from 5 preterm and 5 term neonates. The cells were isolated and characterized as MSCs in accordance with the International Society for Cellular Therapy. We exposed MSCs to different oxygen tensions to examine the impact of environmental factors on cell performance. We studied the following stem cell properties: (i) motility, (ii) proliferation, (iii) senescence, (iv) cell viability, (v) colony-forming unit efficiency, and (vi) inflammatory cytokine expression. Under normoxia (21% O2), cells from preterm and term infants had similar properties. Under hypoxic conditions (1% O2), term MSCs had better cell proliferation; however, cells exposed to hyperoxia (90% O2) had the slowest motility and lowest cell viability (p < 0.05). There was no difference in the expression of senescence or cytokine expression between the groups. The term cells demonstrated more colony-forming efficiency than the preterm cells. In sum, our preliminary findings suggest that MSCs derived from term and preterm umbilical cords have similar characteristics, offering the potential of future autologous/allogeneic MSC transplants in neonates.
AB - Mesenchymal stem cells (MSCs) have shown promise as therapeutic agents in treating morbidities associated with premature birth. MSCs derived from the human umbilical cord are easy to isolate and have low immunogenicity and a robust ability to secrete paracrine factors. To date, there are no studies evaluating preterm versus term umbilical cord tissue-derived MSCs. Therefore, our aim was twofold: (1) to compare stem cell properties in preterm versus term MSCs and (2) to examine the impact of oxygen tension on stem cell behavior. Umbilical cord tissue was obtained from 5 preterm and 5 term neonates. The cells were isolated and characterized as MSCs in accordance with the International Society for Cellular Therapy. We exposed MSCs to different oxygen tensions to examine the impact of environmental factors on cell performance. We studied the following stem cell properties: (i) motility, (ii) proliferation, (iii) senescence, (iv) cell viability, (v) colony-forming unit efficiency, and (vi) inflammatory cytokine expression. Under normoxia (21% O2), cells from preterm and term infants had similar properties. Under hypoxic conditions (1% O2), term MSCs had better cell proliferation; however, cells exposed to hyperoxia (90% O2) had the slowest motility and lowest cell viability (p < 0.05). There was no difference in the expression of senescence or cytokine expression between the groups. The term cells demonstrated more colony-forming efficiency than the preterm cells. In sum, our preliminary findings suggest that MSCs derived from term and preterm umbilical cords have similar characteristics, offering the potential of future autologous/allogeneic MSC transplants in neonates.
UR - http://www.scopus.com/inward/record.url?scp=85049410315&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85049410315&partnerID=8YFLogxK
U2 - 10.1159/000489256
DO - 10.1159/000489256
M3 - Article
C2 - 29949803
AN - SCOPUS:85049410315
SN - 1422-6405
VL - 205
SP - 137
EP - 150
JO - Cells Tissues Organs
JF - Cells Tissues Organs
IS - 3
ER -