Comparison of Palivizumab-Like Antibody Binding to Different Conformations of the RSV F Protein in RSV-Infected Adult Hematopoietic Cell Transplant Recipients

Xunyan Ye, Obinna P. Iwuchukwu, Vasanthi Avadhanula, Letisha O. Aideyan, Trevor J. McBride, Laura L. Ferlic-Stark, Kirtida D. Patel, Felipe Andres Piedra, Dimpy P. Shah, Roy F. Chemaly, Pedro A. Piedra

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Background Most respiratory syncytial virus (RSV) vaccine candidates include fusion (F) protein in different conformations. Antigenic site II found in the different F conformations is the target of palivizumab, the only US Food and Drug Administration approved monoclonal antibody (mAb). Serum palivizumab-like antibody (PLA) is a potential serologic correlate of immunity. Our objective was to determine if different conformations of F protein in a palivizumab competitive antibody (PCA) assay affect the PLA concentrations. Methods Four PCA assays were standardized using mAbs. Each contained prefusion, postfusion, or intermediate F forms. PLA concentrations were measured in acute and convalescent sera from 22 RSV/A and 18 RSV/B-infected adult hematopoietic cell transplant (HCT) recipients. PLA concentrations were calculated using a 4-parameter logistic regression model and analyzed for statistical significance. Results PCA assays revealed significantly greater PLA concentrations in convalescent sera; comparable increases in PLA concentration in RSV/A and RSV/B-infected HCT recipients; and significantly reduced PLA concentrations in HCT recipients who shed RSV ≥14 days. A significant positive correlation was observed between PCA assays and RSV neutralizing antibody titers. Conclusions F protein conformation does not appear to have a measurable impact on PCA assays for measuring PLA induced by RSV/A or RSV/B infection.

Original languageEnglish (US)
Pages (from-to)1247-1256
Number of pages10
JournalJournal of Infectious Diseases
Volume217
Issue number8
DOIs
StatePublished - Mar 28 2018

Keywords

  • RSV
  • Respiratory syncytial virus
  • fusion protein
  • palivizumab competitive antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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