Comparison of low-density lipoprotein size by polyacrylamide tube gel electrophoresis and polyacrylamide gradient gel electrophoresis

Shaina V. Hirany, Yusra Othman, Patricia Kutscher, David L. Rainwater, Ishwarlal Jialal, Sridevi Devaraj

    Research output: Contribution to journalArticlepeer-review

    17 Scopus citations

    Abstract

    We evaluated a low-density lipoprotein (LDL) subfraction separation method using polyacrylamide tube gel electrophoresis (PTGE) and compared it with the reference method, polyacrylamide gradient gel electrophoresis (PGGE-REF). Excellent intra-assay and interassay coefficients of variation were obtained (< 4%) for PTGE. For 102 subjects, LDL subclasses correlated most significantly with triglyceride (TG) level, high-density lipoprotein (HDL) cholesterol level, total cholesterol/HDL cholesterol ratio, and non-HDL cholesterol level (P < .05). The distribution of large LDL (76%) was predominant for subjects with low TG levels (< 150 mg/dL [1.69 mmol/L]), while distribution of small LDL (79%) was predominant for subjects with high TG levels (> 200 mg/dL [2.26 mmol/L]). Excellent agreement between the methods was observed (weighted kappa = 0.78). Of 51 samples classified as small, dense LDL by PGGE-REF, none were misclassified as large LDL and 4 as intermediate LDL by PTGE (92% concordance); of 44 samples classified as large LDL by PGGE-REF, 3 were misclassified as small and 7 as intermediate by PTGE (77% concordance). The PTGE method is precise and compares favorably with PGGE-REF. It has the advantage of being simple, less expensive, and more suitable for use in the clinical laboratory.

    Original languageEnglish (US)
    Pages (from-to)439-445
    Number of pages7
    JournalAmerican journal of clinical pathology
    Volume119
    Issue number3
    DOIs
    StatePublished - Mar 1 2003

    Keywords

    • LDL size
    • LDL subfractions
    • Low-density lipoprotein
    • Risk factor
    • Small, dense LDL

    ASJC Scopus subject areas

    • Pathology and Forensic Medicine

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