Comparison of intravitreal and subretinal injection routes for adenoviral vector mediated gene transfer to the mouse retina

M. L. Suber, M. Y. Hurwitz, C. A. Garcia, K. T. Marcus, P. Chéves-Barrios, E. Aguilar-Cordova, F. L. Hurwitz

Research output: Contribution to journalReview articlepeer-review

Abstract

Purpose. Intravitreal and subretinal injections were performed to compare the administration routes and to determine titer effects for adenoviral vector mediated gene transfer to the mouse retina. Methods. A replication deficient adenoviral vector containing the RSV promoter driving the E. coli β-galactocidase gene was injected into the intravitreal and subretinal spaces of one hundred 3 to 4 week old Balb/c mice at concentrations of 3 x 106 to 1 x 101 iu/ml. The eyes were enucleated 72 hours post-injection and β-galactocidase expression was assessed by histochemical examination. Results. Both injection routes resulted in transduction of the gene to the photoreceptors and pigmented epithelium even at low viral titers. However there was a reduced incidence of ocular inflammation and retinal detachment with an intravitreal injection route. Histochemical analysis also demonstrated a more even dispersion of the vector throughout the retina with an intravitreal injection route. Conclusions. Intravitreal injection of an adenoviral vector directed toward the retina was a more efficient and less traumatic method compared to the subretinal injection route. Adenoviral vectors were able to penetrate the neural retina and pigmented epithelium at a high rate while the integrity of the retina remained intact. This should prove to be a good system for the transduction of genes to the retina.

Original languageEnglish (US)
Pages (from-to)S785
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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