Comparison of inoculation methods for testing enterococci by using vancomycin screening agar

James H. Jorgensen; M. Leticia Mcelmeel; Christa W. Trippy

doi:10.1128/jcm.34.11.2841-2842.1996

Comparison of inoculation methods for testing enterococci by using vancomycin screening agar

James H. Jorgensen, M. Leticia Mcelmeel, Christa W. Trippy

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

One hundred four recent clinical isolates of Enterococcus species were screened for vancomycin resistance by using inocula of 10⁵ or 10⁶ CFU dispensed by pipet and by use of a cotton swab dipped in a 0.5 McFarland standard organism suspension applied to the surface of brain heart infusion agar containing 6 μg of vancomycin per ml. The three inoculation methods were equivalent in the detection of nonsusceptible isolates. The use of swab inoculation was convenient and less costly than the use of micropipets.

Original language	English (US)
Pages (from-to)	2841-2842
Number of pages	2
Journal	Journal of clinical microbiology
Volume	34
Issue number	11
DOIs	https://doi.org/10.1128/jcm.34.11.2841-2842.1996
State	Published - Nov 1996
Externally published	Yes

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/jcm.34.11.2841-2842.1996

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AU - Trippy, Christa W.

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AB - One hundred four recent clinical isolates of Enterococcus species were screened for vancomycin resistance by using inocula of 105 or 106 CFU dispensed by pipet and by use of a cotton swab dipped in a 0.5 McFarland standard organism suspension applied to the surface of brain heart infusion agar containing 6 μg of vancomycin per ml. The three inoculation methods were equivalent in the detection of nonsusceptible isolates. The use of swab inoculation was convenient and less costly than the use of micropipets.

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Comparison of inoculation methods for testing enterococci by using vancomycin screening agar

Abstract

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