Comparison of in-office dental unit waterline test kits

Barry F. Morris, Kraig S. Vandewalle, Donna M. Hensley, Joseph A. Bartoloni

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The authors conducted a study to determine the validity of two commercially available in-office dental unit waterline test kits compared to the gold standard, R2A agar. Samples were collected from the air/water syringes of dental units and cultured on HPC Samplers, Petrifilm AC Plates, and R2A agar plates. HPC Samplers and R2A agar plates were incubated for 7 days and counted manually using magnification. Petrifilm AC Plates were counted after incubation time of 5 and 7 days using an electronic-plate reader. Validity measurements were calculated using a cutoff value ≤500 colony-forming units per milliliter. The accuracy for the HPC Sampler compared to R2A agar was 71%. The accuracy for the Petrifilm AC Plates at 5 and 7 days was 79% and 87% compared to R2A agar. The Petrifilm AC Plate (7-day incubation) demonstrated higher sensitivity, specificity, predictive values, and accuracy than the HPC Sampler kit. Copyright (c) Association of Military Surgeons of the US. All rights reserved.

Original languageEnglish (US)
Pages (from-to)901-906
Number of pages6
JournalMilitary medicine
Issue number11
StatePublished - Nov 2010

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health


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