Abstract
The authors conducted a study to determine the validity of two commercially available in-office dental unit waterline test kits compared to the gold standard, R2A agar. Samples were collected from the air/water syringes of dental units and cultured on HPC Samplers, Petrifilm AC Plates, and R2A agar plates. HPC Samplers and R2A agar plates were incubated for 7 days and counted manually using magnification. Petrifilm AC Plates were counted after incubation time of 5 and 7 days using an electronic-plate reader. Validity measurements were calculated using a cutoff value ≤500 colony-forming units per milliliter. The accuracy for the HPC Sampler compared to R2A agar was 71%. The accuracy for the Petrifilm AC Plates at 5 and 7 days was 79% and 87% compared to R2A agar. The Petrifilm AC Plate (7-day incubation) demonstrated higher sensitivity, specificity, predictive values, and accuracy than the HPC Sampler kit. Copyright (c) Association of Military Surgeons of the US. All rights reserved.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 901-906 |
| Number of pages | 6 |
| Journal | Military Medicine |
| Volume | 175 |
| Issue number | 11 |
| State | Published - Nov 2010 |
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ASJC Scopus subject areas
- Medicine(all)
- Public Health, Environmental and Occupational Health
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Comparison of in-office dental unit waterline test kits. / Morris, Barry F.; Vandewalle, Kraig S.; Hensley, Donna M.; Bartoloni, Joseph A.
In: Military Medicine, Vol. 175, No. 11, 11.2010, p. 901-906.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Comparison of in-office dental unit waterline test kits
AU - Morris, Barry F.
AU - Vandewalle, Kraig S.
AU - Hensley, Donna M.
AU - Bartoloni, Joseph A.
PY - 2010/11
Y1 - 2010/11
N2 - The authors conducted a study to determine the validity of two commercially available in-office dental unit waterline test kits compared to the gold standard, R2A agar. Samples were collected from the air/water syringes of dental units and cultured on HPC Samplers, Petrifilm AC Plates, and R2A agar plates. HPC Samplers and R2A agar plates were incubated for 7 days and counted manually using magnification. Petrifilm AC Plates were counted after incubation time of 5 and 7 days using an electronic-plate reader. Validity measurements were calculated using a cutoff value ≤500 colony-forming units per milliliter. The accuracy for the HPC Sampler compared to R2A agar was 71%. The accuracy for the Petrifilm AC Plates at 5 and 7 days was 79% and 87% compared to R2A agar. The Petrifilm AC Plate (7-day incubation) demonstrated higher sensitivity, specificity, predictive values, and accuracy than the HPC Sampler kit. Copyright (c) Association of Military Surgeons of the US. All rights reserved.
AB - The authors conducted a study to determine the validity of two commercially available in-office dental unit waterline test kits compared to the gold standard, R2A agar. Samples were collected from the air/water syringes of dental units and cultured on HPC Samplers, Petrifilm AC Plates, and R2A agar plates. HPC Samplers and R2A agar plates were incubated for 7 days and counted manually using magnification. Petrifilm AC Plates were counted after incubation time of 5 and 7 days using an electronic-plate reader. Validity measurements were calculated using a cutoff value ≤500 colony-forming units per milliliter. The accuracy for the HPC Sampler compared to R2A agar was 71%. The accuracy for the Petrifilm AC Plates at 5 and 7 days was 79% and 87% compared to R2A agar. The Petrifilm AC Plate (7-day incubation) demonstrated higher sensitivity, specificity, predictive values, and accuracy than the HPC Sampler kit. Copyright (c) Association of Military Surgeons of the US. All rights reserved.
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M3 - Article
C2 - 21121503
AN - SCOPUS:78149273282
VL - 175
SP - 901
EP - 906
JO - Military Medicine
JF - Military Medicine
SN - 0026-4075
IS - 11
ER -