Comparison of chicken erythroid cell nuclear isolation methods using morphological, immunochemical and biochemical criteria

Judith A. Briggs, Milka M. Montiel, Robert C. Briggs, Lubomir S. Hnilica

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Chicken erythroid nuclei were prepared using four published methods. Our findings indicate that nuclei prepared by nitrogen cavitation are less likely to be contaminated with plasma membrane fragments than those made by procedures involving cell disruption by hypotonic lysis. However, globin gene sequences were much less sensitive to DNase I digestion in nuclei prepared by nitrogen cavitation. This suggests that the conformation of chromatin was altered by the cavitation procedure. Analysis of the proteins solubilized during limited DNase I digestion of nuclei prepared by both hypotonic lysis and cavitation revealed no appreciable differences in HMG proteins but a notable difference in the RNP-associated proteins and core histones.

Original languageEnglish (US)
Pages (from-to)29-42
Number of pages14
JournalMolecular and Cellular Biochemistry
Volume74
Issue number1
DOIs
StatePublished - Mar 1987
Externally publishedYes

Keywords

  • DNase I hypersensitivity
  • erythroid nuclei
  • globin c-DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Fingerprint

Dive into the research topics of 'Comparison of chicken erythroid cell nuclear isolation methods using morphological, immunochemical and biochemical criteria'. Together they form a unique fingerprint.

Cite this