Comparison of artery organ culture and co-culture models for studying endothelial cell migration and its effect on smooth muscle cell proliferation and migration

Yong Ung Lee, Jian Luo, Eugene Sprague, Hai Chao Han

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Arterial restenosis associated with intimal hyperplasia is the major cause of long-term failure of vascular interventions. Endothelium injury and the proliferation and migration of smooth muscle cells (SMC) are key events in the development of intimal hyperplasia. The objectives of this study were to develop an ex vivo artery injury model for studying endothelial cell (EC) migration and to compare it with an in vitro co-culture arterial wall injury model in terms of the effect of flow on EC migration and its effect on SMC migration and proliferation. Our results demonstrated that shear flow improves reendothelialization in the injured area by promoting EC migration. The migration distance of ECs is much smaller in the arteries than in an in vitro cell culture model (3.57 ± 1.29 mm vs. 5.2 ± 1.4 cm, p < 0.001). SMC proliferation was significantly less in the EC intact and reendothelialization areas than in the EC denuded areas indicating that reendothelialization suppresses SMC proliferation. Our models provide a new approach to study techniques to enhance endothelium healing.

Original languageEnglish (US)
Pages (from-to)801-812
Number of pages12
JournalAnnals of Biomedical Engineering
Volume38
Issue number3
DOIs
StatePublished - Mar 2010

Keywords

  • Cell culture
  • Cell migration
  • Cell proliferation
  • Organ culture
  • Porcine artery
  • Reendothelialization

ASJC Scopus subject areas

  • Biomedical Engineering

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