Comparative physiological and proteomic analyses reveal the actions of melatonin in the reduction of oxidative stress in Bermuda grass (Cynodon dactylon (L). Pers.)

Haitao Shi, Xin Wang, Dun Xian Tan, Russel J Reiter, Zhulong Chan

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

The fact of melatonin as an important antioxidant in animals led plant researchers to speculate that melatonin also acts in the similar manner in plants. Although melatonin has significant effects on alleviating stress-triggered reactive oxygen species (ROS), the involvement of melatonin in direct oxidative stress and the underlying physiological and molecular mechanisms remain unclear in plants. In this study, we found that exogenous melatonin significantly alleviated hydrogen peroxide (H<inf>2</inf>O<inf>2</inf>)-modulated plant growth, cell damage, and ROS accumulation in Bermuda grass. Additionally, 76 proteins significantly influenced by melatonin during mock or H<inf>2</inf>O<inf>2</inf> treatment were identified by gel-free proteomics using iTRAQ (isobaric tags for relative and absolute quantitation). Metabolic pathway analysis showed that several pathways were markedly enhanced by melatonin and H<inf>2</inf>O<inf>2</inf> treatments, including polyamine metabolism, ribosome pathway, major carbohydrate metabolism, photosynthesis, redox, and amino acid metabolism. Taken together, this study provides more comprehensive insights into the physiological and molecular mechanisms of melatonin in Bermuda grass responses to direct oxidative stress. This may relate to the activation of antioxidants, modulation of metabolic pathways, and extensive proteome reprograming.

Original languageEnglish (US)
Pages (from-to)120-131
Number of pages12
JournalJournal of Pineal Research
Volume59
Issue number1
DOIs
StatePublished - Aug 1 2015

Keywords

  • Bermuda grass
  • iTRAQ (isobaric tags for relative and absolute quantitation)latonin
  • oxidative stress
  • proteomic
  • reactive oxygen species

ASJC Scopus subject areas

  • Endocrinology

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