Abstract
Comparative sequence analysis and ERNA-3D software were used to model the three-dimensional structures of several signal recognition particle RNAs. RNA secondary structures were established by allowing only phylogenetically-supported base pairs. The folding of the RNA molecules was constrained further to include a pseudoknot and a tertiary interaction. Founded by the concept that all SRP RNAs must be shaped similarly in three dimensions, helical sections were oriented coaxially where a continuous helical stack was formed in the RNA of another species. Finally, RNA helices were placed at distances that preserved the connectivity of the molecule with the smallest number of single-stranded nucleotide residues as identified from the aligned sequences. Representative models of the three-dimensional structures of an eukaryote, an archaeon, and three bacterial SRP RNAs are presented.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 405-413 |
| Number of pages | 9 |
| Journal | ACS Symposium Series |
| Volume | 682 |
| State | Published - 1998 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Chemistry(all)
Cite this
Comparative Modeling of the Three-Dimensional Structure of Signal Recognition Particle RNA. / Zwieb, Christian W; Gowda, Krishne; Larsen, Niels; Müller, Florian.
In: ACS Symposium Series, Vol. 682, 1998, p. 405-413.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative Modeling of the Three-Dimensional Structure of Signal Recognition Particle RNA
AU - Zwieb, Christian W
AU - Gowda, Krishne
AU - Larsen, Niels
AU - Müller, Florian
PY - 1998
Y1 - 1998
N2 - Comparative sequence analysis and ERNA-3D software were used to model the three-dimensional structures of several signal recognition particle RNAs. RNA secondary structures were established by allowing only phylogenetically-supported base pairs. The folding of the RNA molecules was constrained further to include a pseudoknot and a tertiary interaction. Founded by the concept that all SRP RNAs must be shaped similarly in three dimensions, helical sections were oriented coaxially where a continuous helical stack was formed in the RNA of another species. Finally, RNA helices were placed at distances that preserved the connectivity of the molecule with the smallest number of single-stranded nucleotide residues as identified from the aligned sequences. Representative models of the three-dimensional structures of an eukaryote, an archaeon, and three bacterial SRP RNAs are presented.
AB - Comparative sequence analysis and ERNA-3D software were used to model the three-dimensional structures of several signal recognition particle RNAs. RNA secondary structures were established by allowing only phylogenetically-supported base pairs. The folding of the RNA molecules was constrained further to include a pseudoknot and a tertiary interaction. Founded by the concept that all SRP RNAs must be shaped similarly in three dimensions, helical sections were oriented coaxially where a continuous helical stack was formed in the RNA of another species. Finally, RNA helices were placed at distances that preserved the connectivity of the molecule with the smallest number of single-stranded nucleotide residues as identified from the aligned sequences. Representative models of the three-dimensional structures of an eukaryote, an archaeon, and three bacterial SRP RNAs are presented.
UR - http://www.scopus.com/inward/record.url?scp=0346614804&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0346614804&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0346614804
VL - 682
SP - 405
EP - 413
JO - ACS Symposium Series
JF - ACS Symposium Series
SN - 0097-6156
ER -