Comparative evaluation of the Limulus assay and the direct gram stain for detection of significant bacteriuria

J. H. Jorgensen, P. M. Jones

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

A double blind study comparing the Limulus in vitro endotoxin assay with the direct Gram stain of uncentrifuged urine for detection of significant bacteriuria was performed. A total of 1077 urine specimens were examined by the two methods and the results compared with results of quantitative urine cultures. 203 samples produced growth of >105 organisms per ml urine. The Limulus assay detected 86.2% of these specimens, and 98.8% of urines that contained >105 Gram negative bacilli per ml. The Gram stain procedure detected only 69.5% of urines containing >105 organisms per ml and 74.5% of specimens with >105 Gram negative bacteria per ml urine. The Limulus assay demonstrated both greater sensitivity and greater specificity than the Gram stain procedure. Moreover, the Limulus test is much less susceptible to errors of interpretation than methods involving microscopy.

Original languageEnglish (US)
Pages (from-to)142-148
Number of pages7
JournalAmerican Journal of Clinical Pathology
Volume63
Issue number1
DOIs
StatePublished - Jan 1 1975

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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