Abstract
As an Old World nonhuman primate, baboons have been extensively used for research on dyslipidemia and atherogenesis. With increasing knowledge about the endothelium's role in the initiation and progression of atherosclerosis, the value of the baboon model can be increased by developing it for research on the role of dysfunctional endothelium in atherogenesis. Toward that goal, we have established and validated methods of isolating and culturing baboon femoral artery endothelial cells (BFAECs) and compared baboon endothelial cellular characteristics with those of humans. Our results indicated that baboon and human endothelial cells share similar growth and culture behaviors. As was the case for human endothelial cells, BFAECs responded to tumor necrosis factor (TNF)-α stimulation with increased expression of adhesion molecules (maximum increase for intracellular adhesion molecule (ICAM): 1.76 ± 0.26-fold; vascular cell adhesion molecule (VCAM): 1.65 ± 0.25-fold; E-selectin: 2.86 ± 0.57-fold). However, BFAECs were hyporesponsive to lipopolysaccharide (LPS) (range, 0.25-20 μg/mL) in adhesion molecule expression, whereas 1 μg/mL LPS induced 2.14- to 3.71-fold increases in human endothelial cells. The differential responses to LPS were not related to TLR-2 and toll-like receptor (TLR)-4 expression on the cell surface. And baboon microvascular endothelial cells had similar features as BFAECs. We observed constitutive expression of interleukin (IL)-6, IL-8, granulocyte macrophage colony-stimulating factor (GM-CSF), and monocyte chemoattractant protein (MCP)-1 in both human and baboon endothelial cells, and these cytokines were further induced by TNF-α and LPS. We also demonstrated that the responses to TNF-α or LPS varied among baboons maintained under the same dietary and environmental conditions, suggesting that response may be controlled by genetic factors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 289-303 |
| Number of pages | 15 |
| Journal | Cell Biochemistry and Biophysics |
| Volume | 40 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 2004 |
| Externally published | Yes |
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Keywords
- Adhesion molecules
- Baboon
- Cytokines
- Endothelial cells
- Femoral artery
ASJC Scopus subject areas
- Cell Biology
- Clinical Biochemistry
- Biophysics
Cite this
Comparative analysis of vascular endothelial cell activation by TNF-α and LPS in humans and baboons. / Shi, Qiang; Wang, Jian; Wang, Xing Li; VandeBerg, John L.
In: Cell Biochemistry and Biophysics, Vol. 40, No. 3, 06.2004, p. 289-303.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Comparative analysis of vascular endothelial cell activation by TNF-α and LPS in humans and baboons
AU - Shi, Qiang
AU - Wang, Jian
AU - Wang, Xing Li
AU - VandeBerg, John L.
PY - 2004/6
Y1 - 2004/6
N2 - As an Old World nonhuman primate, baboons have been extensively used for research on dyslipidemia and atherogenesis. With increasing knowledge about the endothelium's role in the initiation and progression of atherosclerosis, the value of the baboon model can be increased by developing it for research on the role of dysfunctional endothelium in atherogenesis. Toward that goal, we have established and validated methods of isolating and culturing baboon femoral artery endothelial cells (BFAECs) and compared baboon endothelial cellular characteristics with those of humans. Our results indicated that baboon and human endothelial cells share similar growth and culture behaviors. As was the case for human endothelial cells, BFAECs responded to tumor necrosis factor (TNF)-α stimulation with increased expression of adhesion molecules (maximum increase for intracellular adhesion molecule (ICAM): 1.76 ± 0.26-fold; vascular cell adhesion molecule (VCAM): 1.65 ± 0.25-fold; E-selectin: 2.86 ± 0.57-fold). However, BFAECs were hyporesponsive to lipopolysaccharide (LPS) (range, 0.25-20 μg/mL) in adhesion molecule expression, whereas 1 μg/mL LPS induced 2.14- to 3.71-fold increases in human endothelial cells. The differential responses to LPS were not related to TLR-2 and toll-like receptor (TLR)-4 expression on the cell surface. And baboon microvascular endothelial cells had similar features as BFAECs. We observed constitutive expression of interleukin (IL)-6, IL-8, granulocyte macrophage colony-stimulating factor (GM-CSF), and monocyte chemoattractant protein (MCP)-1 in both human and baboon endothelial cells, and these cytokines were further induced by TNF-α and LPS. We also demonstrated that the responses to TNF-α or LPS varied among baboons maintained under the same dietary and environmental conditions, suggesting that response may be controlled by genetic factors.
AB - As an Old World nonhuman primate, baboons have been extensively used for research on dyslipidemia and atherogenesis. With increasing knowledge about the endothelium's role in the initiation and progression of atherosclerosis, the value of the baboon model can be increased by developing it for research on the role of dysfunctional endothelium in atherogenesis. Toward that goal, we have established and validated methods of isolating and culturing baboon femoral artery endothelial cells (BFAECs) and compared baboon endothelial cellular characteristics with those of humans. Our results indicated that baboon and human endothelial cells share similar growth and culture behaviors. As was the case for human endothelial cells, BFAECs responded to tumor necrosis factor (TNF)-α stimulation with increased expression of adhesion molecules (maximum increase for intracellular adhesion molecule (ICAM): 1.76 ± 0.26-fold; vascular cell adhesion molecule (VCAM): 1.65 ± 0.25-fold; E-selectin: 2.86 ± 0.57-fold). However, BFAECs were hyporesponsive to lipopolysaccharide (LPS) (range, 0.25-20 μg/mL) in adhesion molecule expression, whereas 1 μg/mL LPS induced 2.14- to 3.71-fold increases in human endothelial cells. The differential responses to LPS were not related to TLR-2 and toll-like receptor (TLR)-4 expression on the cell surface. And baboon microvascular endothelial cells had similar features as BFAECs. We observed constitutive expression of interleukin (IL)-6, IL-8, granulocyte macrophage colony-stimulating factor (GM-CSF), and monocyte chemoattractant protein (MCP)-1 in both human and baboon endothelial cells, and these cytokines were further induced by TNF-α and LPS. We also demonstrated that the responses to TNF-α or LPS varied among baboons maintained under the same dietary and environmental conditions, suggesting that response may be controlled by genetic factors.
KW - Adhesion molecules
KW - Baboon
KW - Cytokines
KW - Endothelial cells
KW - Femoral artery
UR - http://www.scopus.com/inward/record.url?scp=3943070751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3943070751&partnerID=8YFLogxK
U2 - 10.1385/CBB:40:3:289
DO - 10.1385/CBB:40:3:289
M3 - Article
C2 - 15211029
AN - SCOPUS:3943070751
VL - 40
SP - 289
EP - 303
JO - Cell Biochemistry and Biophysics
JF - Cell Biochemistry and Biophysics
SN - 1085-9195
IS - 3
ER -