TY - JOUR
T1 - Comparative analysis of the central CCK system in Fawn Hooded and Wistar Kyoto rats
T2 - Extended localisation of CCK-A receptors throughout the rat brain using a novel radioligand
AU - Lodge, D. J.
AU - Lawrence, A. J.
N1 - Funding Information:
The authors wish to acknowledge discussions with Prof. P.M. Beart and thank Dr. L. Iversen for the generous gifts of l -364,718 and l -365,260. This work was supported by the Australian Brewer's Foundation and the National Health and Medical Research Council (Australia), of which A.J. Lawrence is a RD Wright Fellow.
PY - 2001/6/15
Y1 - 2001/6/15
N2 - The neuropeptide cholecystokinin has been implicated in the actions of a number of central processes including anxiety and reward. For this reason, the aim of the present study was to compare the density of CCK-A and -B receptors and the mRNA encoding preproCCK throughout the brains of an alcohol-preferring (Fawn Hooded) rat strain with that of a non-alcohol-preferring (Wistar Kyoto) strain of rat. Our study revealed significant differences with regard to the central CCK system of the FH compared to the WKY rat, including differences in CCK-A receptor binding throughout the dorsal medulla, and altered CCK-B binding density throughout the cerebral cortex and reticular nucleus of the thalamus. The most striking result, given the altered behavioural phenotype of the FH rat, was the 33% lower density of CCKmRNA measured throughout the ventral tegmental area of the FH rat when compared to the WKY. This study also reports on a protocol to utilise a novel radioligand, [125I]-D-Tyr-Gly-A-71378, for autoradiographic detection of CCK-A receptors throughout the rat brain. As previously reported, CCK-A receptors were located throughout the area postrema, interpeduncular nucleus and nucleus tractus solitarii; however, binding to CCK-A receptors was also visualised throughout the medial pre-optic area, the arcuate nucleus and the circumventricular regions of the ventral hypothalamus, regions known to contain CCK-A receptors but which were previously undetectable using autoradiography in rat brain.
AB - The neuropeptide cholecystokinin has been implicated in the actions of a number of central processes including anxiety and reward. For this reason, the aim of the present study was to compare the density of CCK-A and -B receptors and the mRNA encoding preproCCK throughout the brains of an alcohol-preferring (Fawn Hooded) rat strain with that of a non-alcohol-preferring (Wistar Kyoto) strain of rat. Our study revealed significant differences with regard to the central CCK system of the FH compared to the WKY rat, including differences in CCK-A receptor binding throughout the dorsal medulla, and altered CCK-B binding density throughout the cerebral cortex and reticular nucleus of the thalamus. The most striking result, given the altered behavioural phenotype of the FH rat, was the 33% lower density of CCKmRNA measured throughout the ventral tegmental area of the FH rat when compared to the WKY. This study also reports on a protocol to utilise a novel radioligand, [125I]-D-Tyr-Gly-A-71378, for autoradiographic detection of CCK-A receptors throughout the rat brain. As previously reported, CCK-A receptors were located throughout the area postrema, interpeduncular nucleus and nucleus tractus solitarii; however, binding to CCK-A receptors was also visualised throughout the medial pre-optic area, the arcuate nucleus and the circumventricular regions of the ventral hypothalamus, regions known to contain CCK-A receptors but which were previously undetectable using autoradiography in rat brain.
KW - A-71378
KW - Alcohol-preferring rat
KW - Autoradiography
KW - CCK receptors
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U2 - 10.1016/S0167-0115(01)00256-7
DO - 10.1016/S0167-0115(01)00256-7
M3 - Article
C2 - 11384782
AN - SCOPUS:0035876855
SN - 0167-0115
VL - 99
SP - 191
EP - 201
JO - Regulatory Peptides
JF - Regulatory Peptides
IS - 2-3
ER -