Collecting mouse livers for transcriptome analysis of daily rhythms

Thomas Mortimer, Patrick Simon Welz, Salvador Aznar Benitah, Kevin B. Koronowski

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular daily rhythms can be captured by precisely timed tissue harvests from groups of animals. This protocol will allow the investigator to identify transcriptional rhythms in the mouse liver while also providing a template for similar analyses in other whole metabolic organs. We describe steps for mouse entrainment, liver dissection, and rhythmicity analysis from total RNA sequencing data. The resulting rhythmic transcriptome will provide the user with a starting point for defining specific biological processes that undergo daily rhythms. For complete details on the use and execution of this protocol, please refer to Koronowski et al. (2019). A similar protocol for interfollicular epidermal cells is demonstrated in Welz et al. (2019).

Original languageEnglish (US)
Article number100539
JournalSTAR Protocols
Volume2
Issue number2
DOIs
StatePublished - Jun 18 2021
Externally publishedYes

Keywords

  • Cancer
  • Metabolism
  • RNA-seq

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Medicine(all)

Fingerprint

Dive into the research topics of 'Collecting mouse livers for transcriptome analysis of daily rhythms'. Together they form a unique fingerprint.

Cite this