@article{d028c15f288746e68d7e6abb727acb13,
title = "Collecting mouse livers for transcriptome analysis of daily rhythms",
abstract = "Molecular daily rhythms can be captured by precisely timed tissue harvests from groups of animals. This protocol will allow the investigator to identify transcriptional rhythms in the mouse liver while also providing a template for similar analyses in other whole metabolic organs. We describe steps for mouse entrainment, liver dissection, and rhythmicity analysis from total RNA sequencing data. The resulting rhythmic transcriptome will provide the user with a starting point for defining specific biological processes that undergo daily rhythms. For complete details on the use and execution of this protocol, please refer to Koronowski et al. (2019). A similar protocol for interfollicular epidermal cells is demonstrated in Welz et al. (2019).",
keywords = "Cancer, Metabolism, RNA-seq",
author = "Thomas Mortimer and Welz, {Patrick Simon} and Benitah, {Salvador Aznar} and Koronowski, {Kevin B.}",
note = "Funding Information: P.-S.W. was supported by a Ramon y Cajal Fellowship from the Spanish Ministry for Science and Innovation (MICINN). Research in the S.A.B. lab is supported by the European Research Council (ERC Advanced Grant), the Government of Catalu{\~n}a (SGR grant), the Government of Spain (MINECO), the La Marat{\'o}/TV3 Foundation, the Spanish Association for Cancer Research (AECC), the Foundation Lilliane Bettencourt, the Axa/Atresmedia Award, and the Worldwide Cancer Research Foundation (WCRF). The IRB Barcelona is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). K.B.K. was supported by NIH-NIDDK (award F32DK121425). T.M. received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant agreement No. 754510. The graphical abstract was created with BioRender.com. K.B.K. and T.M. wrote the manuscript; all authors reviewed, edited, and approved the manuscript. The authors declare no competing interests. Funding Information: P.-S.W. was supported by a Ramon y Cajal Fellowship from the Spanish Ministry for Science and Innovation (MICINN). Research in the S.A.B. lab is supported by the European Research Council (ERC Advanced Grant), the Government of Catalu{\~n}a (SGR grant), the Government of Spain ( MINECO ), the La Marat{\'o}/TV3 Foundation , the Spanish Association for Cancer Research (AECC), the Foundation Lilliane Bettencourt , the Axa/Atresmedia Award , and the Worldwide Cancer Research Foundation (WCRF). The IRB Barcelona is a Severo Ochoa Center of Excellence (MINECO award SEV-2015-0505). K.B.K. was supported by NIH- NIDDK (award F32DK121425). T.M. received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant agreement No. 754510 . The graphical abstract was created with BioRender.com . Publisher Copyright: {\textcopyright} 2021 The Author(s)",
year = "2021",
month = jun,
day = "18",
doi = "10.1016/j.xpro.2021.100539",
language = "English (US)",
volume = "2",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",
}